Molecular cloning of AbGst1 encoding a glutathione transferase differentially expressed during exposure of Alternaria brassicicola to isothiocyanates

FEMS Microbiology Letters
Adnane SellamPhilippe Simoneau

Abstract

The AbGst1 gene encoding a glutathione transferase from the necrotrophic pathogen Alternaria brassicicola was cloned from a benzyl isothiocyanate-treated conidial culture using differential display reverse transcription. The deduced amino-acid sequence of AbGst1p showed a significant degree of similarity to glutathione transferase-I from Saccharomyces cerevisiae and glutathione transferase-III from Schizosaccharomyces pombe. The transcription of AbGst1 was significantly enhanced by isothiocyanates, heavy metals and 1-chloro-2,4-dinitrobenzene. However, no significant transcript response was obtained with superoxide-generating menadione and paraquat. Recombinant AbGst1p expressed in Escherichia coli exhibited high transferase activity with allyl and benzyl isothiocyanates as substrate compared with 1-chloro-2,4-dinitrobenzene, but no peroxidase activity was detected. AbGst1 was upregulated in planta during the first day postinfection, suggesting the potential involvement of this enzyme in isothiocyanate detoxification mechanisms during host plant infection.

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Jan 1, 1983·Critical Reviews in Food Science and Nutrition·G R FenwickW J Mullin
Mar 1, 1981·Progress in Cardiovascular Diseases·A J Liedtke
Aug 1, 1995·The Journal of Applied Bacteriology·A D Brabban, C Edwards
Mar 25, 1995·Nucleic Acids Research·P D SiebertS A Lukyanov
Jan 1, 1997·Proteins·G ChelvanayagamP G Board
May 15, 1997·The Biochemical Journal·C A DowdD Sheehan
Feb 25, 1998·Biochemical and Biophysical Research Communications·J M TregerK McEntee
Nov 12, 1998·Trends in Biochemical Sciences·F JeanmouginT J Gibson
Sep 4, 1999·The Plant Journal : for Cell and Molecular Biology·B P ThommaW F Broekaert
Jun 14, 2002·The Journal of Biological Chemistry·Elizabeth A VealBrian A Morgan
Jul 23, 2003·Analytical Biochemistry·Richard A Bundey, Paul A Insel
Jan 21, 2004·Fungal Genetics and Biology : FG & B·Robert A Cramer, Christopher B Lawrence
Mar 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ute WittstockHeiko Vogel
Jun 12, 2004·Plant Physiology and Biochemistry : PPB·Claire Gachon, Patrick Saindrenan
Feb 5, 2005·FEMS Yeast Research·Peter H NguyenAles Vancura
Feb 18, 2005·Archives of Insect Biochemistry and Physiology·Frédéric FrancisEric Haubruge
Sep 17, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Gemma ThorntonNic Jones

❮ Previous
Next ❯

Citations

Nov 8, 2014·Microbiology·Virginie DufourChristine Baysse
Jan 25, 2011·Microbiological Research·María Elena Báez-FloresMartín Ernesto Tiznado-Hernández
Jan 10, 2019·Frontiers in Plant Science·Gábor GullnerPeter Schröder
Dec 12, 2020·Frontiers in Plant Science·Toby E Newman, Mark C Derbyshire
May 18, 2021·Frontiers in Plant Science·Nathaniel M WestrickMehdi Kabbage

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.