PMID: 9540858Apr 16, 1998Paper

Molecular cloning of acid alpha-glucosidase cDNA of Japanese quail (Coturnix coturnix japonica) and the lack of its mRNA in acid maltase deficient quails

Biochimica Et Biophysica Acta
R KunitaT Kikuchi

Abstract

Acid alpha-glucosidase (GAA) hydrolyzes alpha-1, 4 and alpha-1, 6 glucosidic linkages of oligosaccharides and degrades glycogen in the lysosomes. The full-length GAA I cDNA, pQAM8, was isolated from a cDNA library derived from Japanese quail liver. The cDNA is 3569 base pairs long and has an open reading frame capable of coding 932 amino acids. The deduced amino acid sequence shares 52% identity with human GAA. Transfection of expression vector pETAM8 into COS-7 cells or acid maltase deficient (AMD) quail embryonic fibroblasts increased the level of GAA 20-50-fold. Compared to normal quail, the levels of GAA I mRNA were significantly reduced in the muscle, liver, heart, and brain of AMD quails, suggesting the GAA deficiency in AMD quail is due to a lack of GAA I mRNA. A second GAA II cDNA was identified after probing the cDNA library from the ovarian large follicles of quails with a PCR product derived from cultured quail skin fibroblasts. This clone having 3.1 kb insert, has GAA activity as well (3 to 10 fold increase). This cDNA, designated GAA II, predicted an 873 amino acid polypeptide showing 63% identity to human GAA and 51% identity to the GAA I. The RT-PCR analysis demonstrated that GAA II mRNAs were barely detectable i...Continue Reading

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Citations

Feb 12, 2013·Journal of Molecular Evolution·Marek Gabriško
Jun 19, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Takuya SatohTakao Ojima
Oct 22, 2003·Molecular Genetics and Metabolism·N RabenP H Plotz
May 16, 2019·PeerJ·Yan-Hong Chen, Huabin Zhao
Dec 7, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C D Warren, J Alroy
Aug 24, 2021·Journal of Wildlife Diseases·Robert D JollyKim M Hemsley

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