PMID: 10361714Jun 11, 1999

Molecular cloning of an immunogenic and acid-induced isocitrate dehydrogenase gene from Coxiella burnetii

FEMS Microbiology Letters
S Van NguyenK Hirai

Abstract

The Coxiella burnetii icd gene encoding an immunogenic dimeric NADP(+)-dependent isocitrate dehydrogenase (IDH) was cloned by screening a C. burnetii genomic library with a human positive serum and sequenced. The predicted gene product consists of 427 amino acids (M(r) = 46,600) and showed high identity to the IDHs of Escherichia coli (74%), Salmonella enterica (73%) and IDH-I of Vibrio sp. (71%). The cloned gene complemented an icd-defective E. coli mutant producing a recombinant IDH that had the same biochemical properties as the enzyme from purified C. burnetii. Unlike the homologs from other bacteria, the cloned enzyme was expressed to the highest level in low pH conditions. This distinct property of the cloned IDH suggests that C. burnetii icd gene may have a role in the adaptation of the organism to the harsh acidic environment of the eucaryotic phagolysosomes.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1991·Journal of Bacteriology·J W Foster, H K Hall
Jan 1, 1990·Annals of the New York Academy of Sciences·J M BlondeauThomas J Marrie
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Oct 1, 1996·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R Ohman, M Ridell
Dec 1, 1996·FEMS Microbiology Letters·H A Thompson, M L Suhan

Related Concepts

Enzyme Induction
Genes, Bacterial
Cis-Trans Test
Hydrogen-Ion Concentration
Isocitrate Dehydrogenase-I
Restriction Mapping
Coxiella burnetii
Sequence Determinations, DNA

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