Molecular cloning of Ancient Egyptian mummy DNA
Artificial mummification was practised in Egypt from approximately 2600 BC until the fourth century AD. Because of the dry Egyptian climate, however, there are also many natural mummies preserved from earlier as well as later times. To elucidate whether this unique source of ancient human remains can be used for molecular genetic analyses, 23 mummies were investigated for DNA content. One 2,400-yr-old mummy of a child was found to contain DNA that could be molecularly cloned in a plasmid vector. I report here that one such clone contains two members of the Alu family of human repetitive DNA sequences, as detected by DNA hybridizations and nucleotide sequencing. These analyses show that substantial pieces of mummy DNA (3.4 kilobases) can be cloned and that the DNA fragments seem to contain little or no modifications introduced postmortem.
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Amplification of DNA of Xanthomonas axonopodis pv. citri from historic citrus canker herbarium specimens
Microwave-assisted digestion combined with silica-based spin column for DNA isolation from human bones
Amplification of herpes simplex virus type 1 DNA in human geniculate ganglia from formalin-fixed, nonembedded temporal bones
Biological Sexing of a 4000-Year-Old Egyptian Mummy Head to Assess the Potential of Nuclear DNA Recovery from the Most Damaged and Limited Forensic Specimens
Microsatellite genotyping of medieval cattle from central Italy suggests an old origin of Chianina and Romagnola cattle
Preservation of pathological tissue specimens by freeze-drying for immunohistochemical staining and various molecular biological analyses
Anatomical, cellular and molecular analysis of 8,000-yr-old human brain tissue from the Windover archaeological site
DNA extraction from bovine mummified fetuses and detection of factor XI gene deficiency in the mummies
Ancient Egyptian mummy genomes suggest an increase of Sub-Saharan African ancestry in post-Roman periods
SPLATCHE3: simulation of serial genetic data under spatially explicit evolutionary scenarios including long-distance dispersal
Ten years of the horse reference genome: insights into equine biology, domestication and population dynamics in the post-genome era
Hybridization and polymerase chain reaction amplification of simple repeated DNA sequences for the analysis of forensic stains
Screening methods for detection of ancient Mycobacterium tuberculosis complex fingerprints in next-generation sequencing data derived from skeletal samples
Assessing Metagenomic Signals Recovered from Lyuba, a 42,000-Year-Old Permafrost-Preserved Woolly Mammoth Calf
Nuclear gene sequences and DNA variation of Cryptomeria japonica samples from the postglacial period
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