Molecular cloning of bullfrog corticotropin-releasing factor (CRF): effect of homologous CRF on the release of TSH from pituitary cells in vitro

General and Comparative Endocrinology
Yoichi ItoSakaé Kikuyama

Abstract

Corticotropin-releasing factor (CRF) plays multiple roles in vertebrate species. In non-mammalian vertebrates, CRF has been considered to be the major thyrotropin (TSH)-releasing factor. This notion, however, was derived from experimental data on CRF of mammalian origin. Moreover, in the case of amphibians it has never been directly proved that CRF stimulates the release of TSH from the pituitary. The presently described experiment was conducted to provide direct evidence that homologous CRF enhances the release of TSH from the bullfrog (Rana catesbeiana) pituitary. First, cloning of cDNA encoding bullfrog CRF (fCRF) was accomplished. The cDNA encoding fCRF precursor was isolated from a cDNA library of the bullfrog hypothalamus. The amino acid sequence of fCRF predicted from the amplified cDNA sequence showed 83 and 95% identities with the sequences of ovine and human CRFs, respectively. An antiserum against the fCRF synthesized on the basis of the amino acid sequence was raised and used for immunohistochemical staining of the hypothalamus-hypophyseal region of the bullfrog brain. It stained some of the cell bodies situated mainly in the preoptic area, the nucleus infundibularis dorsalis and nucleus hypothalamicus ventralis and...Continue Reading

References

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Citations

Feb 9, 2010·General and Comparative Endocrinology·Sakae Kikuyama
Oct 22, 2008·Genesis : the Journal of Genetics and Development·Mathilde Paris, Vincent Laudet
May 22, 2009·Annals of the New York Academy of Sciences·Reiko OkadaSakae Kikuyama
Jan 18, 2006·General and Comparative Endocrinology·Graham C Boorse, Robert J Denver
Dec 31, 2005·General and Comparative Endocrinology·Yoichi ItoSakaé Kikuyama
Dec 13, 2005·General and Comparative Endocrinology·Bert De GroefEduard R Kühn
Sep 16, 2014·Frontiers in Endocrinology·Diana C Castañeda CortésJuan I Fernandino
Aug 30, 2016·General and Comparative Endocrinology·Reiko OkadaSakae Kikuyama
Jan 2, 2021·Molecular and Cellular Endocrinology·Sakae KikuyamaReiko Okada

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