Molecular cloning of bullfrog prolactin receptor cDNA: changes in prolactin receptor mRNA level during metamorphosis

General and Comparative Endocrinology
Itaru HasunumaS Kikuyama

Abstract

In amphibian larvae, prolactin (PRL) is known to possess growth-promoting and anti-metamorphic activities. For further understanding of the role of PRL in larvae, bullfrog PRL receptor (bfPRLR) cDNA was obtained from the tail fin of premetamorphic tadpoles by use of the reverse transcription-polymerase chain reaction (RT-PCR) coupled with 5' - and 3' -rapid amplification of cDNA ends (RACE). The predicted bfPRLR was composed of 617 amino acids, contained a single transmembrane domain, and showed 33-57% sequence homologies with known sequences of vertebrate PRLRs. When bfPRLR was transiently expressed, specific binding of 125I-labeled bullfrog PRL (bfPRL) was observed. By Northern blot analysis, a 3-kb transcript was detected in the tail fin. By RT-PCR bfPRLR mRNA expression was detected mainly in the brain, kidney, skin, and tail throughout prometamorphic and middle climactic periods. The results of an RNase protection assay revealed that the bfPRLR mRNA level in the tail fin increased around the onset of climax (stage XX) and was maintained at a relatively high value at least until mid-climax (stage XXII). It also revealed that bfPRLR mRNA level in the kidney of larvae gradually rose as metamorphosis progressed. The results su...Continue Reading

References

Apr 1, 1977·General and Comparative Endocrinology·G K Clemons, C S Nicoll
Nov 1, 1979·General and Comparative Endocrinology·P M Mondou, J C Kaltenbach
Oct 30, 1992·Biochemical and Biophysical Research Communications·M TanakaK Nakashima
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M MurakamiT Kishimoto
Jul 12, 1991·Biochimica Et Biophysica Acta·T KobayashiS Kikuyama
Dec 1, 1990·Journal of Molecular Endocrinology·N TakahashiY Kato
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M EderyJ Djiane
Oct 1, 1972·General and Comparative Endocrinology·K YoshizatoI Yasumasu
Sep 1, 1971·Developmental Biology·R K Hunt, M Jacobson
Feb 1, 1981·Endocrinologia Japonica·K Yamamoto, S Kikuyama
Apr 1, 1982·Endocrinologia Japonica·K Yamamoto, S Kikuyama
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·O SandraP A Kelly
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L Buckbinder, D D Brown
Sep 1, 1994·The Journal of Experimental Zoology·A OguchiS Kikuyama
Mar 1, 1994·Biochimica Et Biophysica Acta·Y B Shi, V C Liang
Jan 1, 1993·Recent Progress in Hormone Research·P A KellyJ Finidori
Jan 1, 1993·International Review of Cytology·S KikuyamaK Yamamoto
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·H Huang, D D Brown
Jun 1, 2000·Development, Growth & Differentiation·T YamamotoA Kawahara
Feb 13, 2001·General and Comparative Endocrinology·C R SantosD M Power
May 29, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroshi MatsukawaSakae Kikuyama
Jan 1, 1946·The Anatomical Record·A C TAYLOR, J J KOLLROS

❮ Previous
Next ❯

Citations

Jan 26, 2008·Zoological Science·Yuki OshimaMasahisa Nakamura
Nov 4, 2015·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Qiaomu HuHanbing Xiao
Jan 17, 2012·General and Comparative Endocrinology·Carmen ClappGonzalo Martínez de la Escalera
Oct 20, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kuo-Wei LiChing-Feng Weng
Jan 2, 2021·Molecular and Cellular Endocrinology·Sakae KikuyamaReiko Okada

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.