Molecular cloning of cDNA encoding human lanosterol synthase

Biological & Pharmaceutical Bulletin
C K SungYutaka Ebizuka

Abstract

A cDNA encoding human lanosterol synthase, the enzyme responsible for the backbone formation step in sterol biosynthesis, was cloned by extensive application of PCRs. Five degenerate oligonucleotide primers (139S, 440S, 528A, 575A and 712A) corresponding to the homologous amino acid sequences among the known 2,3-oxidosqualene cyclase(OSC) were designed. PCR with one pair(440S and 528A) of five primers yielded a 285-bp fragment. PCRs with the primers based on the obtained fragment and the degenerate primers (139S and 712A) gave longer fragments. Finally, full nucleotide sequence of cDNA was obtained by a "rapid amplification of cDNA ends" (RACE) method.

Citations

Aug 4, 2001·Biochemical and Biophysical Research Communications·Y SakakuraK Inoue
Feb 10, 2004·Biochemical and Biophysical Research Communications·Armin RufRalf Thoma
May 13, 2015·Protein Expression and Purification·Charlotte KürtenPer-Olof Syrén
Jan 31, 2004·Phytochemistry·Ran XuSeiichi P T Matsuda
Jan 21, 2006·Angewandte Chemie·Quanbo XiongSeiichi P T Matsuda
Oct 3, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Simonetta Oliaro-BossoGianni Balliano
Oct 30, 2004·Chembiochem : a European Journal of Chemical Biology·Silvia LodeiroSeiichi P T Matsuda
Mar 15, 2006·Plant & Cell Physiology·Masashi SuzukiYutaka Ebizuka
Jun 28, 2003·Natural Product Reports·Michael J R SeguraSeiichi P T Matsuda
Feb 3, 2005·Biological & Pharmaceutical Bulletin·Yuichi SakanoYutaka Ebizuka
Jan 12, 1999·Phytochemistry·J B HerreraS P Matsuda
Jun 19, 2001·Organic Letters·B M JoubertS P Matsuda
Feb 1, 2006·Archives of Biochemistry and Biophysics·Mariya D KolesnikovaSeiichi P T Matsuda

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