PMID: 11319166Apr 25, 2001Paper

Molecular cloning of chicken vasoactive intestinal polypeptide receptor complementary DNA, tissue distribution and chromosomal localization

Biology of Reproduction
N KansakuD Zadworny

Abstract

Chicken vasoactive intestinal polypeptide receptor (VIPR) cDNA was cloned by the reverse transcription-polymerase chain reaction method using primers designed on the basis of other species of VIPR cDNA. The cDNA obtained was sequenced by the dideoxy-mediated chain-termination method. Of the 2227 nucleotides that were sequenced, 84, 855, and 1338 bases represent the 5'-untranslated region (UTR), the 3'-UTR, and the open reading frame that predicts a peptide of 446 amino acids. The cDNA of the chicken VIPR shows 65% and 60% homologies to human cDNA of VIP1 and VIP2 receptors, respectively. The clone had the expected similarity to highly conserved features of the other G protein-coupled receptors (GPCRs) such as six cysteine residues that are functionally important in the VIPR subfamily. In addition, the seven potential membrane-spanning domains characteristic of the family B group III GPCR superfamily and highly conserved motif within the third cellular loop between transmembrane regions 5 and 6. Northern blot hybridization analysis in this study indicated mRNA expression of VIPRs in the various tissues of the chicken. Strong signal was detected in the brain and anterior pituitary gland. High levels of VIPR mRNA in the brain was ...Continue Reading

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Citations

Feb 13, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Tetsuya TachibanaMitsuhiro Furuse
Jan 17, 2013·General and Comparative Endocrinology·Md Sakirul Islam KhanTetsuya Tachibana
Sep 26, 2007·Developmental and Comparative Immunology·Alberto B SilvaDonald B Palmer
Aug 5, 2016·Journal of Zhejiang University. Science. B·Yue-Jin PuYan-Zhang Gong

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