PMID: 9654739Jul 9, 1998Paper

Molecular cloning of chitinase cDNAs from the silkworm, Bombyx mori and the fall webworm, Hyphantria cunea

Insect Biochemistry and Molecular Biology
M G KimH Y Park

Abstract

cDNAs encoding chitinases were cloned and characterized from Bombyx mori and Hyphantria cunea, and their gene expression during the metamorphosis was also studied. The chitinase cDNA from B. mori encodes a protein of 565 amino acids with a calculated molecular mass of 63.4 kDa and the H. cunea chitinase cDNA encodes a protein of 553 amino acids with a calculated molecular mass of 62.0 kDa. Amino acid alignment of the two chitinases revealed 75% homology and 77-80% with M. sexta chitinase. The putative cleavage site of the signal peptide was between amino acid residues 20 and 21 for both chitinases. There were three potential N-glycosylation sites in the chitinase of B. mori at the amino acid residues 86-89, NFTS 304-307, NATG, 398-401, NYTV, whereas two potential N-glycosylation sites were present at the amino acid residues 86-89, NFTA and 304-307, NATG, in that of H. cunea. Southern blot analysis of total genomic DNA suggested that the B. mori genome has only one chitinase gene detectable by the cDNA probe and the H. cunea genome has one or two chitinase gene copies. Northern analysis indicated that gene expression was up-regulated during the molting process, larval-pupal transformation and pupal-adult transformation, when enz...Continue Reading

References

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Citations

Feb 27, 2001·Insect Biochemistry and Molecular Biology·S TsuzukiS Sakurai
Jan 5, 2002·Insect Biochemistry and Molecular Biology·Michiyoshi TakahashiManabu Kamimura
Nov 14, 2002·Insect Biochemistry and Molecular Biology·Y ZhengQ Feng
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Jul 25, 2003·Insect Biochemistry and Molecular Biology·Takaaki DaimonToru Shimada
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Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ji Hee HanByung Rae Jin
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Dec 18, 2013·Archives of Insect Biochemistry and Physiology·Weiwei ZhuoShiqing Xu
Sep 5, 2014·Archives of Insect Biochemistry and Physiology·Pu Yang, Xiao-Ming Chen
Mar 18, 2008·Insect Biochemistry and Molecular Biology·Qingsong ZhuSubbaratnam Muthukrishnan
Jan 28, 2015·Insect Biochemistry and Molecular Biology·Daqi LiJianzhen Zhang
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