Molecular cloning of DNAs encoding the regulatory subunits of elongin from Saccharomyces cerevisiae and Drosophila melanogaster

Biochemical and Biophysical Research Communications
T Aso, M N Conrad

Abstract

The Elongin complex strongly stimulates the rate of elongation by RNA polymerase II by suppressing transient pausing by polymerase at many sites along the DNA. Elongin is composed of a transcriptionally active A subunit and two positive regulatory B and C subunits. The Elongin complex is a potential target for regulation by the von Hippel-Lindau (VHL) tumor suppressor protein, which is capable of binding stably to the Elongin BC complex and preventing it from activating Elongin A. Here, we report the molecular cloning of a Saccharomyces cerevisiae genomic DNA encoding Elongin C subunit and of Drosophila cDNAs encoding Elongin B and C subunits. The predicted amino acid sequence of each protein shows a high degree of similarity with the mammalian proteins. The recombinant yeast Elongin C protein interacts with both mammalian Elongin A and VHL tumor suppressor protein. Moreover, yeast Elongin C strongly induces the transcriptional elongation activity of mammalian Elongin A. The expression of yeast Elongin C mRNA is dramatically upregulated during sporulation; however, the gene is not essential for sporulation and viability in yeast cell.

Citations

Jul 26, 2013·Molecular Biology of the Cell·Wojciech StecMartin P Zeidler
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·M V BotuyanW J Chazin
Mar 11, 2009·DNA Repair·Anne Daulny, William P Tansey
Sep 8, 2001·Journal of Molecular Biology·M V BotuyanC H Arrowsmith
Feb 28, 2002·The Journal of Biological Chemistry·Linda E HymanWilliam H Baricos
Feb 7, 2001·The Journal of Biological Chemistry·C M KothA M Edwards
Dec 22, 2019·Neuroscience and Biobehavioral Reviews·Christine B SiebergDavid Borsook
Apr 13, 2000·Biochimica Et Biophysica Acta·T JacksonL E Hyman
Aug 29, 2003·Biochemical and Biophysical Research Communications·Kenji TamuraTeijiro Aso

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