PMID: 2504277May 16, 1989Paper

Molecular cloning of dog mast cell tryptase and a related protease: structural evidence of a unique mode of serine protease activation

Biochemistry
P VandersliceGeorge H Caughey

Abstract

Mast cell tryptase is a secretory granule associated serine protease with trypsin-like specificity released extracellularly during mast cell degranulation. To determine the full primary structure of the catalytic domain and precursor forms of tryptase and to gain insight into its mode of activation, we cloned cDNAs coding for the complete amino acid sequence of dog mast cell tryptase and a second, possibly related, serine protease. Using RNA from dog mastocytoma cells, we constructed a cDNA library in lambda gt 10. Screening of the library with an oligonucleotide probe based on the N-terminal sequence of tryptase purified from the same cell source allowed us to isolate and sequence overlapping clones coding for dog mast cell tryptase. The tryptase sequence includes the essential residues of the catalytic triad and an aspartic acid at the base of the putative substrate binding pocket that confers P1 Arg and Lys specificity on tryptic serine proteases. The apparent N-terminal signal/activation peptide terminates in a glycine. A glycine in this position has not been observed previously in serine proteases and suggests a novel mode of activation. Additional screening of the library with a trypsinogen cDNA led to the isolation and s...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1989·The Journal of Clinical Investigation·K SekizawaJ A Nadel
Mar 1, 1989·Journal of Cellular Biochemistry·J R VasquezC S Craik
Jan 1, 1986·Annual Review of Biochemistry·R A PadgettP A Sharp
Mar 22, 1985·Science·D J Lipman, W R Pearson
Nov 1, 1987·Archives of Biochemistry and Biophysics·G H CaugheyJ A Nadel
Nov 3, 1987·Biochemistry·H Le TrongR G Woodbury
Aug 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G H CaugheyW M Gold
Oct 18, 1988·Biochemistry·S J RemingtonH Neurath
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K GershenfeldI L Weissman
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D JenneJ Tschopp
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jun 1, 1985·Archives of Biochemistry and Biophysics·H KidoN Katunuma
Aug 13, 1985·Biochemistry·A L TarentinoT H Plummer
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Mar 25, 1983·Nucleic Acids Research·L DenteR Cortese
Jun 10, 1983·Science·C S CraikR Fletterick
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G H SwiftR J MacDonald

❮ Previous
Next ❯

Citations

Mar 1, 1992·Protein Science : a Publication of the Protein Society·D A Johnson, G J Barton
Dec 3, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M J CaoT Ishihara
Jan 25, 2003·European Journal of Biochemistry·Alessandra GambacurtaFranca Ascoli
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P VandersliceG H Caughey
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S ReynoldsW E Serafin
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H P McNeilR L Stevens
Jan 1, 1997·Mediators of Inflammation·M Q Zhang, H Timmerman
Jun 11, 1992·Nucleic Acids Research·E D LampertiL Villa-Komaroff
Nov 1, 1993·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·G A StewartA S McWilliam
Jan 1, 1991·Annals of the New York Academy of Sciences·J A Nadel
Apr 17, 2013·Biochemistry. Biokhimii︠a︡·T S Zamolodchikova
May 1, 1991·American Journal of Respiratory Cell and Molecular Biology·G H Caughey
Nov 26, 2009·American Journal of Respiratory Cell and Molecular Biology·Neil N Trivedi, George H Caughey
Oct 1, 1989·The Journal of Clinical Investigation·J S MillerL B Schwartz
Sep 1, 1990·The Journal of Clinical Investigation·J S MillerL B Schwartz
Apr 5, 2003·Biological Chemistry·Masayuki SatoMakoto Yamashita
Apr 17, 1995·FEBS Letters·L FiorucciF Ascoli
May 15, 2007·Immunological Reviews·George H Caughey
Feb 15, 2005·Archives of Biochemistry and Biophysics·Wilfred W RaymondGeorge H Caughey
May 11, 2015·European Journal of Pharmacology·George H Caughey
May 29, 1990·Biochemistry·G H CaugheyP Vanderslice
May 21, 1991·Biochemistry·V J Braganza, W H Simmons
Mar 16, 1993·Biochemistry·N M SchechterD R McCaslin
Jun 23, 1998·The Journal of Biological Chemistry·P J WoltersG H Caughey
Jun 2, 1995·The Journal of Biological Chemistry·W W RaymondG H Caughey
May 17, 2007·The Journal of Biological Chemistry·H Patrick McNeilRichard L Stevens
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G H CaugheyG M Verghese
Feb 2, 1996·The Journal of Biological Chemistry·J E HuntN Ghildyal
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M B RaoV V Deshpande

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved