PMID: 9185665Jun 1, 1997Paper

Molecular cloning of Fyn-associated molecules in the mouse central nervous system

Journal of Neuroscience Research
N KaiT Yagi

Abstract

Fyn tyrosine kinase is expressed extensively in the central nervous system (CNS) of mammals, and its genetic disruption in mouse displays several behavioral abnormalities with morphological and electrophysiological defects in the brain. To understand the signaling pathways in which Fyn is involved in the CNS, we screened molecules that directly associate with Fyn in neonatal mouse brain by using a two-hybrid yeast system. We isolated five cDNA clones with strong and reproducible Fyn-binding activity. Sequence analyses revealed that three of them are previously reported molecules, SON, tctex-1, and hnRNP K, and that two clones encode novel sequences. The hnRNP K has been shown to associate with Fyn, so our yeast system is appropriate to isolate Fyn-binding molecules. Northern hybridization analyses indicated that all isolated clones are expressed in the mouse brain and that the mRNA levels of the two molecules (tctex-1 and clone 82) change during development in the brain. A full-length cDNA of clone 82 was obtained and its deduced amino acid sequence was homologous to the RNA-binding proteins. Isolation of many Fyn-binding molecules suggest that, in the mouse CNS, Fyn mediates multiple signaling pathways by binding to multiple m...Continue Reading

References

Oct 30, 1992·Cell·T Pawson, G D Gish
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K SembaK Toyoshima
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·R J RicklesM J Zoller
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·I M BuyseS Huang
Feb 1, 1995·Brain Research. Molecular Brain Research·T MiyakawaH Niki
Mar 3, 1995·The Journal of Biological Chemistry·T Tomonaga, D Levens
Nov 1, 1994·Brain Research. Molecular Brain Research·T MiyakawaH Niki
Nov 1, 1994·The Journal of Cell Biology·H E BeggsP F Maness
May 1, 1994·Biology of Reproduction·M J BaumV E Papaioannou

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Citations

Apr 23, 2002·Protein Science : a Publication of the Protein Society·Stephen J KingTrina A Schroer
Oct 1, 2005·Molecular Biology of the Cell·Linda M DiBellaStephen M King
Dec 24, 2010·Nucleic Acids Research·Kee K KimSachiyo Kawamoto
Feb 3, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Réda Hadj-SlimanePierre Bobé
Mar 2, 2011·The Journal of Cell Biology·Lisbeth S LaursenCharles Ffrench-Constant
Jan 25, 2005·Journal of Molecular Biology·Markus RalserSylvia Krobitsch
Apr 10, 2004·Neuroreport·Shigeki YuasaTakeshi Yagi
Jun 23, 2011·Reproductive Sciences·Xin HuangQing-Yuan Sun
Jul 21, 1999·Nature Neuroscience·P Mombaerts
Oct 9, 2018·Annual Review of Cell and Developmental Biology·George MountoufarisTom Maniatis
Sep 30, 2006·Cell Motility and the Cytoskeleton·David E WilkesDavid J Asai
Feb 11, 2005·The Journal of Biological Chemistry·John C WilliamsWayne A Hendrickson
Nov 10, 2007·Reviews in Medical Virology·Russell J DiefenbachAnthony L Cunningham
Jul 4, 1998·The Journal of Biological Chemistry·T Y HsiehM M Lai

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