PMID: 418411Mar 1, 1978

Molecular cloning of genetically active fragments of Bacillus DNA in Bacillus subtilis and properties of the vector plasmid pUB110

Proceedings of the National Academy of Sciences of the United States of America
K M KegginsE J Duvall

Abstract

Plasmid pUB110 (approximately 2.8 x 10(6) daltons), originally detected in Staphylococcus aureus, specifies resistance to neomycin and has been transformed into Bacillus subtilis 168, In B. subtilis, pUB110 is stably maintained at about 50 copies per chromosome and renders the host resistant to neomycin sulfate at 5 microgram/ml. pUB110 isolated from B. subtilis transforms Rec+ and recE4-containing strains of B. subtilis at frequencies greater than or equal to 10(3) transformants per microgram of plasmid. pUB110 was transferred by PBS1 or SP10 transduction from B. subtilis to strains of B. pumilus and B. licheniformis. pUB110 is compatible with each of four previously described Bacillus plasmids, including pPL576, pPL10, pPL7065, and pPL2. pUB110 contains a single EcoR1-sensitive site and was used as vector to clone DNA fragments that complement the trpC2 mutation in B. subtilis 168 from EcoR1 digests of the chromosome DNA isolated from B. pumilus strains NRRL B-3275 and NRS576, B. licheniformis strains 9945A and 749C, and B. subtilis 168. Genetic and physical properties of each of the constructed Trp derivatives of pUB110 are described.

References

Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S D Ehrlich
Sep 1, 1977·Antimicrobial Agents and Chemotherapy·P S LovettR Taylor
Jan 25, 1975·Journal of Molecular Biology·M Thomas, R W Davis
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·L Clarke, J Carbon
Nov 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S N CohenR B Helling
Aug 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D DubnauJ Marmur
Jun 27, 1969·Biochemical and Biophysical Research Communications·S O HochI P Crawford
May 25, 1966·Biochemical and Biophysical Research Communications·I H Goldberg, K Mitsugi
Feb 1, 1965·Journal of Molecular Biology·F W STUDIER
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

Citations

Jan 1, 1981·Molecular & General Genetics : MGG·C Tackney, Rivka Rudner
Dec 1, 1987·Molecular & General Genetics : MGG·K B Temeyer, L F Chapman
Jan 1, 1986·Molecular & General Genetics : MGG·R E MullerS Aiba
Jan 1, 1982·Molecular & General Genetics : MGG·G MazzaS Riva
Sep 1, 1979·Molecular & General Genetics : MGG·T Tanaka
May 1, 1981·Mikrobiologiia·F G KupriianovaV G Vinter
Jan 1, 1981·Molecular & General Genetics : MGG·J I Yoder, A T Ganesan
Mar 1, 1986·Microbial Ecology·E J Stellwag, J E Brenchley
Jan 1, 1980·The International Journal of Biochemistry·N K Pandey
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·T J Gryczan, D Dubnau
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Winston, N Sueoka
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D L StassiS A Lacks
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R KornN Sueoka
Aug 11, 1982·Nucleic Acids Research·J SmithL D Bell
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·K TsugeM Shoda
Apr 1, 1985·Canadian Journal of Microbiology·T HoshinoN Tomizuka
Jan 11, 2012·Biotechnology and Bioengineering·Qingzhao WangJauhleene Chamu
Nov 5, 2014·Letters in Applied Microbiology·R HertelH Liesegang
Dec 3, 2009·Biotechnology Progress·Chung-Jen ChiangYun-Peng Chao
Jul 1, 1981·Plasmid·J Scheer-AbramowitzD Dubnau
Jun 1, 1978·Plasmid·A G Shivakumar, D Dubnau
Jan 1, 1983·Annals of the New York Academy of Sciences·S AibaJ Koizumi
Jan 1, 1981·Annals of the New York Academy of Sciences·D H Dean, M J Kaelbling
Jan 1, 1983·CRC Critical Reviews in Biochemistry·W V Shaw
Nov 1, 1979·CRC Critical Reviews in Biochemistry·D R Helinski
Jun 14, 2006·Regulatory Toxicology and Pharmacology : RTP·Zofia S Olempska-BeerMichael J DiNovi
Mar 5, 2016·AMB Express·Geomárcia Feitosa da Cruz RamosSuzan Pantaroto de Vasconcellos
Jan 1, 1980·CRC Critical Reviews in Biochemistry·I P Crawford
Jul 11, 2017·Frontiers in Microbiology·Younju SoSoo-Keun Choi
Feb 16, 2017·Applied Microbiology and Biotechnology·Sen YangZhen Kang
Dec 7, 2018·BMC Microbiology·Timothy D ReadMichael Z David

Related Concepts

Natto Bacteria
DNA Restriction Enzymes
DNA, Bacterial
Recombination Joint
Antibiotic Resistance, Microbial
Cis-Trans Test
Neomycin Palmitate
Episomes
Transduction, Genetic
Transformation, Genetic

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
T J Gryczan, D Dubnau
Proceedings of the National Academy of Sciences of the United States of America
S D Ehrlich
Proceedings of the National Academy of Sciences of the United States of America
S D Ehrlich
© 2021 Meta ULC. All rights reserved