PMID: 15226597Jul 1, 2004Paper

Molecular cloning of goat 20alpha-hydroxysteroid dehydrogenase cDNA

The Journal of Reproduction and Development
Walimuni Samantha Nilanthi JayasekaraMasugi Nishihara

Abstract

20Alpha-hydroxysteroid dehydrogenase (20alpha-HSD), which catalyzes the conversion of progesterone to its inactive form 20alpha-dihydroprogesterone, is expressed in murine placenta and has been suggested to play roles in maintaining pregnancy. To understand the role of 20alpha-HSD during pregnancy in the goat, as a first step, cloning and sequencing of 20alpha-HSD cDNA were performed. The full nucleotide sequence of 20alpha-HSD cDNA was determined on samples obtained from the corpus luteum at the luteal phase of the estrous cycle and the placenta in late pregnancy by RT-PCR and 3' and 5' RACE systems. Cloned 20alpha-HSD cDNA consisted of 1124 bp and belonged to the aldo-keto reductase superfamily. From the start codon to stop codon there were 323 amino acids, the same as in other species. To verify whether the protein derived from goat 20alpha-HSD cDNA had 20alpha-HSD activity, the cDNA was expressed by bacteria. Bacterially expressed goat 20alpha-HSD protein showed 20alpha-HSD enzyme activity. A tissue distribution study demonstrated that 20alpha-HSD was expressed in the placenta, but not in the adrenal gland, liver and spleen during pregnancy. The present study suggests that goat 20alpha-HSD is another member of the aldo-keto...Continue Reading

References

May 1, 1990·Journal of Reproduction and Fertility·P HumblotR G Sasser
Dec 1, 1985·Journal of Steroid Biochemistry·J A PinedaJ C Warren
Jun 1, 1972·The Journal of Endocrinology·G IrvingA Knifton
Jan 1, 1972·The Journal of Endocrinology·G D Thorburn, W Schneider
Dec 1, 1993·Endocrine Journal·K ShiotaT Ogawa
Oct 23, 1997·The Biochemical Journal·J M JezT M Penning

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Citations

Sep 13, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Purevjargal NaidansurenKwan-Sik Min
Feb 11, 2005·The Journal of Reproduction and Development·Walimuni Samantha Nilanthi JayasekaraMasugi Nishihara
Aug 1, 2008·The Journal of Reproduction and Development·Jae-hyek ChoiMasugi Nishihara
Jan 27, 2012·Animal Reproduction Science·P J NaidansurenK S Min
Aug 13, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Tsevelmaa NanjidsurenKwan-Sik Min

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