PMID: 11342117May 9, 2001Paper

Molecular cloning of human thiamin pyrophosphokinase

Biochimica Et Biophysica Acta
R ZhaoI D Goldman

Abstract

Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state.

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Citations

Mar 22, 2005·Gene·Satoru IshidaSoichi Takiguchi
Jun 11, 2005·Protein Expression and Purification·Jean-Marc DelroisseMicheline Vandenbol
May 26, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Svetlana M Nabokina, Hamid M Said
Jun 19, 2004·The International Journal of Biochemistry & Cell Biology·Bernard LakayeLucien Bettendorff
Feb 19, 2013·The American Journal of Pathology·Arati SharmaGavin P Robertson
May 19, 2016·Expert Review of Neurotherapeutics·Juan Darío Ortigoza-EscobarBelén Pérez-Dueñas
Apr 24, 2018·Frontiers in Plant Science·Simon Strobbe, Dominique Van Der Straeten
Mar 25, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said
May 17, 2019·Journal of Inherited Metabolic Disease·Anna Marcé-GrauBelén Pérez-Dueñas

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