PMID: 8612620Mar 1, 1996Paper

Molecular cloning of mouse 17 beta-hydroxysteroid dehydrogenase type 1 and characterization of enzyme activity

European Journal of Biochemistry
P NokelainenR Vihko

Abstract

The biological activity of certain estrogens and androgens is modulated by enzymes called 17 beta-hydroxysteroid dehydrogenases (17 beta-HSDs), which catalyze the interconversion between less active 17-oxosteroid and more active 17 beta-hydroxysteroid forms. In the present report, we describe cloning of mouse 17 beta-HSD type-1 cDNA from an ovarian library generated from 4,4'-(1,2-diethyl-1,2-ethenediyl)bisphenol-(diethylstilbestrol)-tr eated mice, and characterization of the corresponding enzyme. The open reading frame of the mouse 17 beta-HSD type-1 cDNA encodes a peptide of 344 amino acid residues with a predicted molecular mass of 36785 Da. The mouse 17 beta-HSD type-1 enzyme shares 63% and 93% overall identity with human and rat 17 beta-HSD type-1 enzymes, respectively, and the most striking differences between the mouse and human type-1 enzymes are between the amino acid residues 197 and 230 and in the carboxy terminus of the enzymes. Similarly to the human 17 beta-HSD type-1 enzyme, the mouse type-1 enzyme primarily catalyzes reductive reactions from 17-oxo forms to 17 beta-hydroxy forms in intact cultured cells, but unlike the human type-1 enzyme, the mouse enzyme does not prefer phenolic over neutral substrates. Thus, ...Continue Reading

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Citations

Mar 18, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski
May 8, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski
Feb 13, 2001·Molecular and Cellular Endocrinology·V Luu-TheF Labrie
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Jun 1, 2001·The Journal of Steroid Biochemistry and Molecular Biology·V Luu-The
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Dec 9, 2008·Molecular and Cellular Endocrinology·Taija SaloniemiMatti Poutanen
Jun 1, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Georges PelletierFernand Labrie
Jul 7, 2009·Molecular and Cellular Endocrinology·Eric BoucherYves Tremblay
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Dec 6, 2008·General and Comparative Endocrinology·Rosalba PuttiMarina Paolucci
Dec 30, 2008·Molecular and Cellular Endocrinology·R Mindnich, J Adamski

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