PMID: 6403942Apr 1, 1983Paper

Molecular cloning of Notch, a locus affecting neurogenesis in Drosophila melanogaster

Proceedings of the National Academy of Sciences of the United States of America
S Artavanis-TsakonasB Yedvobnick

Abstract

The Notch locus is one of the best characterized loci in Drosophila melanogaster in terms of its genetic structure and developmental effects. Mutations in this locus profoundly affect the differentiation of the early embryo. Using an inversion involving the Notch locus and previously cloned sequences, we have isolated chromosomal segments from the Notch region (3C7) encompassing 80 kilobases (kb) of DNA. Based on comparison between mutant and wild-type DNA, we have positioned cloned sequences within the Notch genetic map; furthermore, we have defined a region of approximately 40 kb within which the structural lesions correlating with all Notch alleles mapped to date appear to reside. We have examined the transcriptional activity of the cloned sequences during ontogeny and find a single size class of poly(A)+ RNA, 10.5 kb long, that is homologous to sequences within this 40-kb region. We conclude that DNA sequences belonging to the Notch locus have been cloned and that the 10.5-kb poly(A)+ RNA is essential for wild-type Notch function. We discuss these structural and transcriptional data in light of the existing genetic and developmental characterization of the Notch locus.

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Citations

Jan 1, 1985·Molecular & General Genetics : MGG·M A MortinJ J Bonner
Feb 1, 1985·Developmental Biology·B G GrimwadeS Artavanis-Tsakonas
Jun 1, 1985·Developmental Biology·M RoarkJ A Lengyel
Mar 1, 1989·Developmental Biology·J L DenburgJ A Marner
Oct 9, 2002·Immunological Reviews·David AllmanWarren S Pear
Jan 24, 2006·Nature Reviews. Neuroscience·Angeliki Louvi, Spyros Artavanis-Tsakonas
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A SwaroopA Garen
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G M Technau, J A Campos-Ortega
Apr 17, 2009·Molecular Biology and Evolution·Athina TheodosiouSophia Kossida
Feb 7, 2012·Blood·Anna Bigas, Lluis Espinosa
Nov 17, 2010·BMC Medicine·Maria Maddalena LinoJean-Louis Boulay
May 16, 2013·PloS One·Heather MainEmma R Andersson
Dec 21, 2006·Genetics·Hajime TakeuchiDieter Egli
Jul 13, 2012·Genetics·Iva Greenwald
Nov 3, 2010·Development Genes and Evolution·Megan J WilsonPeter K Dearden
Nov 30, 2012·Annual Review of Physiology·Taeko K Noah, Noah F Shroyer
Jan 20, 2016·Biology·Akihiko Murata, Shin-Ichi Hayashi
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Aug 12, 2005·Annals of the New York Academy of Sciences·Andrew D McCulloch, Giovanni Paternostro
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Aug 4, 2010·IUBMB Life·Zhi-Dong ZhouEng-King Tan
Oct 5, 2011·European Journal of Haematology·Olaf HopferWolf-Karsten Hofmann
Nov 1, 2014·Thoracic Cancer·Huajiao GuoJian Zhang
Aug 29, 2014·Journal of Cellular and Molecular Medicine·Gabriel Rusanescu, Jianren Mao
Sep 1, 1983·Cell·S KiddM W Young

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