PMID: 2502391Jul 1, 1989Paper

Molecular cloning of preproacrosin and analysis of its expression pattern in spermatogenesis

European Journal of Biochemistry
I M AdhamW Engel

Abstract

Complementary DNA clones for the boar preproacrosin have been isolated from a randomly primed testis cDNA library in lambda gt10 and from an oligo(dT)-primed testis cDNA in lambda gt11. The nucleotide sequence of the 1418-bp cDNA insert includes a 46-bp 5'-untranslated region, an open reading frame of 1248 bp corresponding to 416 amino acids (45.59 kDa) and a 121-bp 3'-untranslated region. The deduced amino acid sequence includes the active-site residues histidine, asparagine and serine of the catalytic triad of the serine proteinase super-family and is colinear with that determined by amino acid sequencing of the boar acrosin light chain and of a small region of the NH2-terminal sequence of the heavy chain. The preproacrosin cDNA contains at the 3' end a 381-bp sequence which codes for an amino acid sequence not yet found in any other serine proteinase. This amino acid sequence is rich in proline (42 out of 127 amino acids) and is suggested to be involved in the recognition and binding of the spermatozoa to the zona pellucida of the ovum. The mRNA for preproacrosin is synthesized as an approximately 1.6-kb-long molecule only in the postmeiotic stages of boar and bull spermatogenesis.

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Citations

May 31, 1990·European Journal of Biochemistry·S KeimeW Engel
Jul 1, 1992·European Journal of Biochemistry·M H Vazquez-LevinJ W Gordon
Feb 1, 1990·Differentiation; Research in Biological Diversity·U KlemmW Engel
Jun 12, 2013·Gene·Hai-Tao Mao, Wan-Xi Yang
Jul 20, 2016·Molecular Human Reproduction·A RedaJ-B Stukenborg
Feb 24, 2000·Biology of Reproduction·L I FurlongM H Vazquez-Levin
Nov 30, 1990·Biochemical and Biophysical Research Communications·S KashiwabaraT Baba
Dec 14, 2004·Molecular Human Reproduction·Heike ObermannChristiane Kirchhoff
Apr 1, 1992·Molecular Reproduction and Development·K NayerniaW Engel
Aug 1, 1993·The Anatomical Record·B E KurthJ C Herr
Feb 6, 2008·Molecular Reproduction and Development·Denise Raterman, Mark S Springer
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Oct 8, 1991·Biochimica Et Biophysica Acta·U KlemmW Engel

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