PMID: 9425122Feb 28, 1998Paper

Molecular cloning of rat mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase and detection of the corresponding mRNA and of those encoding the remaining enzymes comprising the ketogenic 3-hydroxy-3-methylglutaryl-CoA cycle in central nervous system of suckling rat

The Biochemical Journal
T E CullingfordJ B Clark

Abstract

We have investigated, by RNase protection assays in rat brain regions and primary cortical astrocyte cultures, the presence of the mRNA species encoding the three mitochondrially located enzymes acetoacetyl-CoA thiolase, mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (mt. HMG-CoA synthase) and HMG-CoA lyase (HMG-CoA lyase) that together constitute the ketogenic HMG-CoA cycle. As a prerequisite we obtained a full-length cDNA encoding rat HMG-CoA lyase by degenerate oligonucleotide-primed PCR coupled to a modification of PCR-rapid amplification of cDNA ends (PCR-RACE). We report here: (1) the nucleotide sequence of rat mt. HMG-CoA lyase, (2) detection of the mRNA species encoding all three HMG-CoA cycle enzymes in all regions of rat brain during suckling, (3) approximately twice the abundance of mt. HMG-CoA synthase mRNA in cerebellum than in cortex in 11-day-old suckling rat pups, (4) significantly lower abundances of mt. HMG-CoA synthase mRNA in brain regions derived from rats weaned to a high-carbohydrate/low-fat diet compared with the corresponding regions derived from the suckling rat, and (5) the presence of mt. HMG-CoA synthase mRNA in primary cultures of neonatal cortical astrocytes at an abundance similar to that ...Continue Reading

Citations

May 2, 2003·Nutritional Neuroscience·Carl E Stafstrom, Kristopher J Bough
Jan 16, 2010·Journal of Toxicology and Environmental Health. Part a·Ameer Y TahaW M Burnham
Aug 30, 2005·Metabolism: Clinical and Experimental·Ameer Y TahaStephen C Cunnane
Aug 28, 2007·Neurochemical Research·Radovan Murín, Bernd Hamprecht
Jul 11, 2009·The Journal of International Medical Research·C YilmazH Caksen
Apr 27, 2002·Annals of the New York Academy of Sciences·Simon Melov
Apr 6, 2017·Frontiers in Endocrinology·Alejandra Freire-RegatilloJulie A Chowen
Sep 14, 2019·The Journal of Biological Chemistry·Andréa HemmerlinThomas J Bach
Jan 30, 2002·Progress in Lipid Research·Simon Eaton
Feb 11, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Toshiyuki FukaoGrant A Mitchell
Feb 11, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Tim E Cullingford

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