Molecular cloning of spermidine/spermine N1-acetyltransferase from the periimplantation porcine uterus by messenger ribonucleic acid differential display: temporal and conceptus-modulated gene expression

Endocrinology
M L GreenR C Simmen

Abstract

Using messenger RNA (mRNA) differential display, we isolated several putative differentially expressed complementary DNAs (cDNAs) from the periimplantation (days 11-12) endometrium of unilaterally pregnant pigs. Nucleotide sequence analysis revealed that one cDNA clone was 87% homologous to human spermidine/ spermine N1-acetyltransferase (SSAT) over a stretch of 201 bp and represents the porcine homologue of this cDNA. A second differentially expressed cDNA encoded the porcine equivalent of the human fragile X mental retardation gene (FMR1), whereas a third specified an open reading frame with significant homology to the Escherichia coli N-acetylglucosamine transfer protein. Because SSAT is the rate-limiting enzyme in polyamine metabolism and polyamines are required cytosolic components for cell growth and differentiation, we characterized the expression of the porcine SSAT gene as a potential marker for endometrial growth and/or differentiation during early pregnancy. Further, using the consensus sequence from human and mouse cDNAs, PCR primers were designed and used to generate a 568-bp cDNA fragment from gravid endometrium that encompassed the entire open reading frame for porcine SSAT and which was subsequently used for Nor...Continue Reading

Citations

Jul 9, 2004·Reproductive Biology and Endocrinology : RB&E·Christopher I Ace, William C Okulicz
Jun 21, 2011·The Journal of Reproduction and Development·Germaine Miese-LooyChandrakant Tayade
Jan 9, 2004·Biology of Reproduction·Andrés A KowalskiRosalia C M Simmen
Dec 6, 2007·Reproduction in Domestic Animals = Zuchthygiene·O Distl
Jul 28, 2011·Endocrine Reviews·Pavine L C LefèvreBruce D Murphy
Jun 8, 2004·Endocrine Reviews·S K DeyHaibin Wang

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