Molecular cloning of ssd-form neural cell adhesion molecules (N-CAMs) as the major form in Xenopus heart

Biochemical and Biophysical Research Communications
M KudoK Shiokawa

Abstract

Different forms of neural cell adhesion molecule (N-CAM) are generated by alternative splicing of primary transcripts and considered to have distinct biological functions. We cloned cDNAs encoding a new form of N-CAMs from the Xenopus heart cDNA library. Comparison of the sequences with chicken and mouse N-CAMs revealed that these clones code for ssd-form N-CAM. We demonstrate by Northern blot analysis that the ssd form is the major form expressed in the Xenopus adult heart. We obtained two types of ssd-form N-CAM, which are transcripts from N-CAM 1 and N-CAM 2 genes. Both types contain muscle specific domain (MSD) but not pi domain. Northern blot analysis also indicated that this form is not expressed in adult brain, in which ld-form N-CAM is the main N-CAM expressed. It is possible that high levels of specific expression of ssd-form N-CAM are related with the differentiation of cardiac muscles.

References

Feb 28, 1992·Biochemical and Biophysical Research Communications·C HessionR Lobb
Jun 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M WatanabeH Fallah-Najmabadi
Mar 1, 1992·The Journal of Cell Biology·P YangU Rutishauser
Jan 1, 1991·Annual Review of Biochemistry·G M Edelman, K L Crossin
Jul 1, 1991·Differentiation; Research in Biological Diversity·P M LackieJ Roth
Jul 1, 1991·The Journal of Cell Biology·A AchesonU Rutishauser
Dec 1, 1989·Cardiovascular Drugs and Therapy·W J Rutishauser
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G C OwensB A Cunningham
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J J HemperlyB A Cunningham
Jan 1, 1985·Annual Review of Biochemistry·G M Edelman
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Nov 15, 1983·Biochemical and Biophysical Research Communications·R K Margolis, R U Margolis
Feb 15, 1994·Journal of Neuroscience Research·R ProbstmeierJ Schneider-Schaulies

❮ Previous
Next ❯

Citations

Jun 18, 2010·Developmental Neurobiology·Elisabeth Rungger-BrändleDuri Rungger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.