Abstract
The human monoclonal antibody AE6F4 specifically reacts with human lung cancer tissues but does not with normal tissues. This monoclonal antibody recognizes a cytosolic 31 kDa antigen in the cancer cells. In a previous study, we elucidated that the 31 kDa antigen belonged to a family of proteins collectively designated as 14-3-3 proteins, which were known as protein kinase-dependent activators of tyrosine/trytophan hydroxylases, or protein kinase C inhibitor proteins. Here we report molecular cloning of the 31 kDa antigen from the human lung adenocarcinoma cell line, A549. Sequencing analysis indicates that the cloned cDNA is identical to that of previously reported human placental cytosolic phospholipase A2 (cPLA2), which is also a member of the 14-3-3 protein family. Western analysis demonstrated that a 31 kDa recombinant cPLA2 expressed in monkey COS cells was recognized by the AE6F4 monoclonal antibody. Binding of the monoclonal antibody to the recombinant cPLA2 was abolished when treated with sodium periodate, suggesting that not only are carbohydrate chains associated with the cPLA2, but they also play a crucial role in antigen recognition by the monoclonal antibody.
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 17, 1977·Science·A UllrichH M Goodman
Mar 26, 1991·Biochimica Et Biophysica Acta·P J Nielsen
Jul 31, 1990·European Journal of Biochemistry·A TokerA Aitken
Apr 15, 1990·The Biochemical Journal·H J GoldbergK L Skorecki
Jan 1, 1989·Biotherapy·S HashizumeK Nomoto
Jan 1, 1989·Advances in Cancer Research·S Hakomori
Jan 1, 1988·Molecular and Cellular Biology·Y TakebeN Arai
Jul 13, 1987·FEBS Letters·T IchimuraH Fujisawa
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R M Burch, J Axelrod
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T IchimuraY Takahashi
Jun 4, 1974·Biochemistry·V GlisinC Byus
Aug 15, 1970·Nature·U K Laemmli
May 1, 1982·Journal of Neurochemistry·P F BostonR J Thompson
May 1, 1982·Journal of Neurochemistry·P F BostonR J Thompson
Jan 29, 1993·Cell·L L LinR J Davis
May 1, 1996·Bioscience, Biotechnology, and Biochemistry·Y InoueS Shirahata
Citations
May 5, 1998·Molecular and Biochemical Parasitology·M Siles-LucasB Gottstein
Nov 1, 1997·Cytotechnology·T SiripenS Shirahata
Jan 1, 1997·Cytotechnology·M YamaguchiS Shirahata
Nov 13, 2008·Cytotechnology·A IchikawaS Shirahata
May 29, 2007·Biochemical Pharmacology·Brian S Cummings
Nov 9, 2011·Journal of the Korean Surgical Society·Young Sun YooKyung Jong Kim
Jan 22, 2011·The Veterinary Journal·Alejandro Suárez-BonnetAntonio Espinosa de los Monteros