Molecular cloning of the cDNA encoding human deoxyribonuclease II.

The Journal of Biological Chemistry
T YasudaK Kishi

Abstract

A rapid amplification of cDNA ends method, using degenerate oligonucleotides based upon the N-terminal amino acid sequence of human hepatic deoxyribonuclease II (DNase II), allowed a novel cDNA encoding DNase II to be constructed from thyroid gland RNA. The composite nucleotide sequence (1593 bases) included an open reading frame of 1080 bases, which encoded a single polypeptide of 360 amino acids (signal peptide, 16; propeptide, 91; mature protein, 253). Although the sequence of DNase II showed no significant homology to other mammalian proteins, its cDNA structural organization resembled those of the lysosomal cathepsin families. The two parts of the cDNA corresponding to the propeptide and the mature protein were expressed in Escherichia coli, and the recombinant polypeptides thus obtained were strongly stained with an anti-DNase II antibody on Western blotting. DNase II is ubiquitously expressed in human tissues, and the DNase II gene (DNASE2) was assigned to chromosome 19.

References

Dec 5, 1991·European Journal of Biochemistry·I HaroshJ B Boyd
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P L FaustJ M Chirgwin
Aug 1, 1968·Canadian Journal of Biochemistry·C Cordonnier, G Bernardi
Jan 1, 1984·The International Journal of Biochemistry·M M OosthuizenJ C Schabort
Dec 1, 1995·The Journal of Biological Chemistry·A TorrigliaM F Counis
Aug 1, 1994·The International Journal of Biochemistry·H TakeshitaK Kishi
Sep 17, 1993·Clinica Chimica Acta; International Journal of Clinical Chemistry·T YasudaK Kishi
Jan 1, 1993·Archives of Biochemistry and Biophysics·M A Barry, A Eastman
Nov 1, 1952·The Journal of General Physiology·V ALLFREY, A E MIRSKY
Feb 1, 1994·Trends in Cell Biology·M C PeitschJ Tschopp

❮ Previous
Next ❯

Citations

Nov 18, 2000·Electrophoresis·A JournetJ Garin
Nov 9, 2001·Biochemical and Biophysical Research Communications·D Shiokawa, S I Tanuma
Dec 4, 2003·Gene·Cory J Evans, Renato J Aguilera
Jun 19, 2001·Biochimica Et Biophysica Acta·S MoriK Kishi
Aug 26, 2003·Legal Medicine·K KishiH Takeshita
Dec 22, 1999·The International Journal of Biochemistry & Cell Biology·Y NakashimaK Kishi
Nov 30, 2004·Nature Immunology·Hideyuki YoshidaShigekazu Nagata
Feb 28, 2003·European Journal of Biochemistry·Haruo TakeshitaKoichiro Kishi
May 9, 2002·Cornea·Shigekazu Nagata
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·N I Aleksandrushkina, B F Vanyushin
May 1, 2001·Biochemical and Biophysical Research Communications·T YasudaK Kishi
Nov 25, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·O HosomiK Kishi
Mar 15, 2005·Inhalation Toxicology·Yuh-Chin T HuangJoleen M Soukup
Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·Kazuo ShiomiHiroshi Nagai
Sep 23, 2006·Biochimie·Marie-France Counis, Alicia Torriglia
Jan 24, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kersti Øverbø, Bjørnar Myrnes
Nov 29, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Junko FujiharaHaruo Takeshita
Mar 29, 2001·Experimental Eye Research·A TorrigliaM F Counis
Aug 28, 2010·Autophagy·Takahiko AkematsuHiroshi Endoh
Aug 29, 2014·PLoS Neglected Tropical Diseases·Chengshui LiaoXiaolei Liu
Aug 14, 1999·The Journal of Biological Chemistry·F Karimi-BusheriM Weinfeld
Jan 31, 2004·Clinical Chemistry·Haruo TakeshitaKoichiro Kishi
Nov 13, 1998·The Journal of Biological Chemistry·R J Krieser, A Eastman

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.