PMID: 8946389Nov 25, 1996Paper

Molecular cloning of the Toxoplasma gondii sag4 gene encoding an 18 kDa bradyzoite specific surface protein

Molecular and Biochemical Parasitology
C Odberg-FerragutJ F Dubremetz

Abstract

An 18 kDa bradyzoite specific surface protein of Toxoplasma gondii (T. gondii) has been purified by affinity chromatography with a specific monoclonal antibody using parasites grown in vitro under conditions inducing the biosynthesis of bradyzoite specific proteins. N-terminal and internal amino acid sequences obtained by microsequencing enabled us to design degenerate oligonucleotides. A fragment of 187 bp was amplified by polymerase chain reaction (PCR). It was used as a probe to clone a 4 kb-Bam HI fragment encompassing the gene encoding the 18 kDa protein. Nucleotide sequence analysis revealed a single open reading frame of 516 nucleotides encoding a 172 amino acid protein. The deduced amino acid sequence matched perfectly the peptides microsequenced from the native protein. The N-terminal hydrophobic region was found to possess the characteristics of a signal peptide of 27 amino acids. The hydrophobic C-terminal part could represent a signal for a glycan-phosphoinositide anchor. The full-length cDNA was also isolated and both the 5' and 3' untranslated regions were determined. Reverse transcriptase-PCR (RT-PCR) using p18-specific primers showed a stage-specific expression of this gene. Comparison of the nucleic acid sequen...Continue Reading

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Citations

Feb 17, 2010·Journal of Ocular Biology, Diseases, and Informatics·Kevin M Brown, Ira J Blader
Sep 22, 2001·International Journal for Parasitology·C LekutisJ C Boothroyd
Mar 21, 1998·International Journal for Parasitology·J C BoothroydI D Manger
May 2, 2002·Trends in Parasitology·Russell E LyonsCraig W Roberts
Dec 11, 2002·Trends in Parasitology·David J P Ferguson, Stephen F Parmley
Mar 22, 2002·The Journal of Eukaryotic Microbiology·C ContiniR Cultrera
Dec 6, 2005·BMC Biology·Jay R RadkeMichael W White
Mar 12, 2009·PloS One·Elizabeth A GaskellGlenn A McConkey
Apr 14, 2000·Frontiers in Bioscience : a Journal and Virtual Library·L M Weiss, K Kim
Feb 14, 2006·Journal of Muscle Research and Cell Motility·Angelika Herm-GötzDominique Soldati
Apr 1, 2018·Cellular and Molecular Life Sciences : CMLS·Victoria JeffersWilliam J Sullivan
Apr 15, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Khadijeh KhanalihaBorna Salemi
Feb 12, 1998·Methods : a Companion to Methods in Enzymology·A HehlJ C Boothroyd
Jul 16, 2021·Frontiers in Immunology·Maguy HamieHiba El Hajj

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