Molecular cloning of the viperin gene and its promoter region from the mandarin fish Siniperca chuatsi

Veterinary Immunology and Immunopathology
B J Sun, P Nie

Abstract

A viperin gene has been cloned from the mandarin fish (Siniperca chuatsi). From the first transcription initiation site, the mandarin fish viperin gene extends 3163 nucleotides to the end of the 3' untranslated region, and it contains six exons and five introns. The open reading frame of the viperin transcript has 1062 nucleotides which encode a 354 amino acid peptide. The amino acid sequence of mandarin fish viperin shows high identities with its homologues in teleosts and mammals except for the first 70 amino acids. A characteristic feature in the viperin promoter region was the presence of five putative ICSBP (IRF8) binding sites and one IRF1 binding site. The viperin gene expressed mainly in lymphoid tissues before stimulation, but its expression can be examined in almost all the organs investigated after stimulation with virus or Poly I:C. The expression pattern and promoter sequence may be considered as the indirect evidence that the transcription of viperin is regulated by interferons or interferon induced genes.

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Citations

Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Gwonhwa SongThomas E Spencer
May 16, 2014·Fish & Shellfish Immunology·Bing WangJian-Fang Gui
Dec 15, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Katherine A Fitzgerald
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Mar 19, 2011·Developmental and Comparative Immunology·Eloi R VerrierPierre Boudinot
Dec 17, 2009·Veterinary Immunology and Immunopathology·Weizhang Jia, Xiuxia Zhou
Jul 25, 2014·Developmental and Comparative Immunology·Bing WangJian-Fang Gui
Nov 12, 2013·Developmental and Comparative Immunology·Ying-Chun YehHan-Ching Wang
Feb 10, 2007·Veterinary Immunology and Immunopathology·B J SunP Nie
Aug 19, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karla J HelbigMichael R Beard
Jul 30, 2018·Fish Physiology and Biochemistry·Xiaowu ChenJinliang Zhao
Mar 22, 2019·Frontiers in Immunology·Khalil EslamlooMatthew L Rise
Jun 12, 2021·Developmental and Comparative Immunology·Yan GaoXue-Qin Liu
Sep 18, 2021·Frontiers in Immunology·Cheng-Yan MouJian-Fang Gui

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