Molecular cloning of TvDAO1, a gene encoding a D-amino acid oxidase from Trigonopsis variabilis and its expression in Saccharomyces cerevisiae and Kluyveromyces lactis

Yeast
F J GonzálezAngel Domínguez

Abstract

The DAO1 gene of Trigonopsis variabilis encoding a D-amino acid oxidase (EC 1.4.3.3) was isolated from genomic clones selected for their specific hybridization to synthetic oligodeoxyribonucleotide probes based on regions of the enzyme that have been conserved through evolution. The nucleotide sequence of the gene predicts a protein with similarities to human, pig, rabbit, mouse and Fusarium solani D-amino acid oxidases. The open reading frame of the T. variabilis DAO1 gene was interrupted by an intron. The Dao1p sequence displays two regions, one in the N-terminal section--the FAD binding site--and the other near the C-terminal region that contains conserved signatures found in all the D-amino acid oxidases. The three C-terminal amino acids suggest that the enzyme may be located in peroxisomes. Northern blot experiments showed that no transcriptional activation occurred in the presence of D-methionine. The cDNA encoding Dao1p was expressed in Saccharomyces cerevisiae and Kluyveromyces lactis. Both yeast species are able to synthesize a functional enzyme under the control of the GAL1 promoter. In K. lactis, up to six times more enzyme units per gram of dry weight are produced with a multicopy plasmid in comparison with the wild...Continue Reading

References

Mar 1, 1976·Analytical Biochemistry·C P Berg, F A Rodden
Sep 1, 1990·Journal of Biochemistry·K MomoiY Miyake
Mar 1, 1989·European Journal of Biochemistry·M Pilone SimonettaS Ronchi
Jun 15, 1989·Biochemical and Biophysical Research Communications·E CiccarelliA Bollen
Dec 1, 1986·Molecular and Cellular Biology·S KimS M Kingsman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Jan 1, 1970·Annual Review of Biochemistry·A H Neims, L Hellerman
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
May 18, 1981·FEBS Letters·M Le Hir, U C Dubach
Apr 24, 1982·Nucleic Acids Research·M J DobsonL A Fothergill
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Nov 26, 1984·Nucleic Acids Research·J L TeemW H Mager
Nov 17, 1995·Cell·R A Rachubinski, S Subramani
Dec 1, 1993·European Journal of Biochemistry·T Schräder, J R Andreesen
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·A MatteviB Curti
Jul 1, 1993·Applied and Environmental Microbiology·M SánchezA Domínguez
Jun 1, 1996·Applied and Environmental Microbiology·R HornerL Fischer

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Citations

Jan 10, 2014·Microbial Cell Factories·Katrin WeinhandlAndrea Camattari
May 2, 2001·Bioscience, Biotechnology, and Biochemistry·H YurimotoN Kato
Apr 28, 2010·Microbial Cell Factories·Sandra AbadAnton Glieder
Dec 5, 2006·The FEBS Journal·Masumi KataneHiroshi Homma
Mar 23, 2011·Enzyme Research·Jessica KopfMiguel Arroyo
Dec 18, 2007·Applied Microbiology and Biotechnology·Loredano PollegioniMirella S Pilone
Feb 11, 2005·Biochemistry. Biokhimii︠a︡·V I Tishkov, S V Khoronenkova
Jan 16, 2014·Bioprocess and Biosystems Engineering·Senwen DengDongzhi Wei
Feb 7, 2007·Applied Microbiology and Biotechnology·Birgit GeuekeWerner Hummel
Oct 4, 2011·Biotechnology Advances·Eda Celik, Pınar Calık

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