PMID: 8612611Mar 1, 1996Paper

Molecular cloning of two different mannose-binding lectins from tulip bulbs

European Journal of Biochemistry
Els J M Van DammeWilly J Peumans

Abstract

Two lectins were isolated from the bulbs of Tulipa cv. Apeldoorn and their corresponding cDNA clones analyzed. The first, called TxLMII (second mannose-binding Tulipa hybrid lectin), is a novel mannose-binding tulip lectin. Based on its molecular structure, carbohydrate-binding specificity and amino acid sequence, TxLMII belongs to the superfamily of mannose-binding monocot lectins which are also found in representatives of the plant families Amaryllidaceae, Alliaceae, Orchidaceae and Araceae. Molecular cloning of the second lectin, called TxLCI (first Tulipa hybrid lectin with complex specificity), allowed determination unambiguously of the molecular structure of this previously described protein. In addition, evidence is presented that each TxLCI subunit possesses a mannose-binding site and an N-acetylgalactosamine-binding site, which act independently of each other. Both binding sites are located in a separate domain of the lectin polypeptide. Since the first domain of TxLCI shows sequence similarity to TxLMII, it is suggested that their genes evolved from a common ancestor.

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Citations

Dec 31, 2004·Journal of Biosciences·Guoyin KaiKexuan Tang
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·T B Ng, Y L Yu
Aug 10, 2000·European Journal of Biochemistry·E J Van DammeW J Peumans
Apr 25, 2001·The Plant Journal : for Cell and Molecular Biology·X WangW Dillen
Nov 25, 2005·Bioscience Reports·Juan LinKexuan Tang
Sep 30, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·L S OoiV E Ooi
Feb 28, 2013·Chemical Society Reviews·Jun HirabayashiHiroaki Tateno
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Sep 27, 2003·DNA Sequence : the Journal of DNA Sequencing and Mapping·Yong-Zhen PangKe-Xuan Tang

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