Molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing carboxylesterase gene from Ochrobactrum anthropi YZ-1

Journal of Hazardous Materials
Yi ZhaiYanchun Yan

Abstract

Strain YZ-1 was isolated from activated sludge and identified as Ochrobactrum anthropi. This strain was capable of degrading pyrethroids pesticides, suggesting the presence of degrading enzymes. In the present study, a novel esterase gene pytZ was cloned from the genomic library of YZ-1 successfully. The pytZ contained an open reading frame of 606bp encoding a pyrethroid-hydrolyzing carboxylesterase. Deduced amino acid sequence showed moderate identities (39-59%) with most homologous carboxylesterase, except a putative carboxylesterase from O. anthropi ATCC 49188 with the highest identity of 85%. Phylogenetic analysis revealed that PytZ belonged to esterase VI family. The gene pytZ showed no any sequence similarity with reported pyrethroid-hydrolyzing genes and was a new pyrethroid-degrading gene. PytZ was expressed in Escherichia coli BL21 (DE3) and purified using Ni-NTA Fast Start. PytZ was able to degrade various pyrethroids. The optimal temperature and pH were 35°C and 7.5. This enzyme was very stable over a wide range of temperature and pH. No cofactors were required for enzyme activity. Broad substrate specificity, high enzyme activity, and the favorable stability make the PytZ a potential candidate for the detoxification...Continue Reading

References

Jan 1, 1992·Archives of Microbiology·S E MaloneyA R Smith
Jun 9, 2004·Applied and Environmental Microbiology·Young J ChoiByong H Lee
Sep 15, 2005·Journal of Agricultural and Food Chemistry·Wei Q LiangYu H Liu
Apr 14, 2007·Biodegradation·Preeti N TallurHarichandra Z Ninnekar
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Feb 9, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Mokhtar I Yousef
Jul 10, 2010·Journal of Environmental Sciences (China)·Quan ZhangMeirong Zhao
Nov 1, 2007·Environmental Toxicology and Pharmacology·Babu VelmuruganErhan Unlu

❮ Previous
Next ❯

Citations

May 21, 2016·Frontiers in Microbiology·Ilse VanhoutteLeen De Gelder
Jun 9, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Shamsa Akbar, Sikander Sultan
Oct 4, 2016·Frontiers in Microbiology·Mariusz Cycoń, Zofia Piotrowska-Seget
Apr 13, 2018·Natural Product Reports·Hideomi ItohYoshitomo Kikuchi
Feb 20, 2018·Frontiers in Microbiology·Hui ZhanShaohua Chen
Aug 2, 2018·Current Microbiology·Xiangxiang HaoKuai Liu
Nov 16, 2014·Applied Microbiology and Biotechnology·Ying XiaoGuohua Zhong
Aug 21, 2019·Frontiers in Microbiology·Pankaj BhattShaohua Chen
Jun 5, 2021·Colloids and Surfaces. B, Biointerfaces·Ummie Umaiera Mohd JohanMohd Shukuri Mohamad Ali
Jun 22, 2021·Journal of Environmental Health Science & Engineering·Lina QiuJiandi Li
Oct 17, 2017·Journal of Agricultural and Food Chemistry·Ai-Xing TangYi-Ming Qing

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.