PMID: 18724689Aug 30, 2008Paper

Molecular cloning, recombinant expression and characterization of lysozyme from Chinese shrimp Fenneropenaeus chinensis

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Xingjiang BuJin-Xing Wang

Abstract

Lysozyme hydrolyses bacterial cell walls and acts as a nonspecific innate immunity molecule against the invasion of bacterial pathogens. We cloned the cDNA of lysozyme from Fenneropenaeus chinensis and named Fc-lysozyme (FcLyz in short). The full length of the gene was of 709 bp, and the open reading frame (477 bp) encoded 158 amino acids. The predicted protein had a signal peptide (-1--18 residue) and molecular weight of the mature protein (residue 1-140) was of 16.2 kD. A Lyz 1 domain (residue 1-130) in the lysozyme was found by SMART analysis. The results of semiquantity RT-PCR showed that FcLyz was constitutively expressed in tested tissues in a low level in normal shrimp, and up-regulated in hemocytes, heart, hepatopancreas and gill of bacterial challenged shrimp. The DNA fragment of mature Fc-Lys was subcloned to pET-30a (+) expression vector, the recombinant plasmid was transformed into Escherichia coli BL21 (DE3) and then induced by isopropylthio-beta-D-galactoside (IPTG). The antibacterial activity of the purified recombinant FcLys was analyzed and minimal inhibitory concentration (MIC) was assayed. The recombinant protein showed high antibacterial activity against some Gram-positive bacteria, and MIC reached 3.43 micr...Continue Reading

Citations

Nov 15, 2011·Fish & Shellfish Immunology·Alma B Peregrino-UriarteRogerio R Sotelo-Mundo
Jul 15, 2015·Molecular Immunology·Xian-Wei Wang, Jin-Xing Wang
Sep 8, 2010·Fish & Shellfish Immunology·Hong-Wei ZhangJin-Xing Wang
Aug 17, 2019·Frontiers in Immunology·Chaozheng LiJianguo He
Feb 22, 2012·Fish & Shellfish Immunology·Feng-ying GaoMaixin Lu

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