Molecular cloning, sequence analysis, expression, and tissue distribution of suppressin, a novel suppressor of cell cycle entry.

The Journal of Biological Chemistry
R D LeBoeufJ D Tauber

Abstract

Suppressin (SPN) is an inhibitor of cell proliferation that was originally identified and purified to homogeneity from bovine pituitaries (LeBoeuf, R. D., Burns, J. N., Bost, K. L., and Blalock, J. E. (1990) J. Biol. Chem. 265, 158-165). In this report we have cloned the full-length cDNA encoding rat SPN and have identified the tissue distribution of SPN expression. The cDNA of SPN is 1882 nucleotides with a 1488-base coding region and 55 and 339 nucleotides of 5'- and 3'-untranslated sequences, respectively. Northern gel analysis of rat pituitary mRNA showed a single hybridizing species at approximately 2 kilobases. Sequence analyses showed that the nucleotide and deduced amino acid sequences of SPN are novel and unrelated to any known vertebrate inhibitors of proliferation. However, the deduced amino acid sequence of SPN contains two domains that have extensive sequence identity with a recently cloned transcription activator in Drosophila, deformed epidermal autoregulatory factor-1 (DEAF-1, see Gross, C. T., and McGinnis, W. (1996) EMBO J. 15, 1961-1970) suggesting that SPN represents a vertebrate cognate of deformed epidermal autoregulatory factor-1. Reverse transcriptase-polymerase chain reaction and immunohistochemical ana...Continue Reading

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Citations

Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Darien E ReedKate Senger
Oct 11, 2011·BMC Genomics·Florbela A VieiraDeborah M Power
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·T M SugiharaB Andersen
Mar 29, 2014·American Journal of Medical Genetics. Part a·Eissa A FaqeihNamik Kaya
Apr 15, 2014·American Journal of Human Genetics·Anneke T Vulto-van SilfhoutBert B A de Vries
Aug 1, 2002·Oncogene·Rebecca B Scarr, Phillip A Sharp
Jul 12, 2002·Genesis : the Journal of Genetics and Development·Alexey VeraksaWilliam McGinnis
May 30, 2007·Oncogene·L B HessonF Latif
Jan 29, 2002·The Journal of Biological Chemistry·Sunil KaulS Stoney Simons
Mar 12, 2003·Oncogene·Angelo AgathanggelouFarida Latif
Mar 12, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shimeng ChenFei Yin

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