PMID: 9171409Jun 1, 1997Paper

Molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus

Journal of Bacteriology
L Ramadurai, R K Jayaswal

Abstract

A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading frame, designated lytM, of 948 bp that could encode a polypeptide of 316 amino acid residues was identified. The calculated molecular mass of the lytM gene product (34.4 kDa) corresponded to that of the autolytic activity detected (approximately 36 kDa) in the Lyt- mutant. Results deduced from amino acid sequence analysis and N-terminal amino acid sequencing data suggest that LytM is a secreted protein. The C-terminal region of the putative protein encoded by lytM showed 51% identity with the N-terminal region of the mature lysostaphin from Staphylococcus simulans and 50% identity with the N-terminal region of ALE-1 from Staphylococcus capitis EPK1. Northern blot analysis showed that lytM expresses a transcript of approximately 955 bp, as predicted from the DNA sequence. Escherichia coli clones carrying the lytM gene exhibited autolytic-activity bands of approximately 36 kDa as well as of 19 and 22 kDa in activity gels. The lytM gene was mapped to the SmaI-D fra...Continue Reading

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Citations

Feb 14, 2006·Proteomics·Christine L GatlinScott N Peterson
Apr 30, 2009·Molecular Microbiology·Sandro F F PereiraAlexander Tomasz
May 15, 2012·Journal of Basic Microbiology·Mu ChunhuaYang Guang
Jun 7, 2003·Molecular Microbiology·S S IngavaleA L Cheung
Mar 29, 2014·International Journal of Microbiology·Vineet K Singh
Jun 29, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Elzbieta JagielskaIzabela Sabała
Aug 27, 2010·FEMS Microbiology Letters·Vineet K SinghKuldeep Singh
Oct 19, 2012·Applied Microbiology and Biotechnology·Piotr SzwedaSlawomir Milewski
Jan 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Samuel BeckerDominique Missiakas
Feb 7, 2007·Genes & Genetic Systems·Takayuki NambuKazuhiro Kutsukake
Oct 7, 2015·Scientific Reports·Maja GrabowskaIzabela Sabala
Jul 21, 2017·Clinical Microbiology Reviews·Elisabeth HodilleOana Dumitrescu
Feb 12, 2015·FEMS Microbiology Letters·Daniel C Osipovitch, Karl E Griswold
May 7, 2003·Applied and Environmental Microbiology·Trine NilsenHelge Holo
Jan 5, 2005·Journal of Bacteriology·Tamaki FujiwaraMotoyuki Sugai
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Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·W W Navarre, O Schneewind
Oct 12, 2021·Frontiers in Microbiology·Alicja WysockaIzabela Sabała

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