Molecular cloning, tissue expression and regulation of nutrition and temperature on Δ6 fatty acyl desaturase-like gene in the red claw crayfish (Cherax quadricarinatus)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Dong-Lei WuYunlong Zhao

Abstract

Desaturase enzymes play an important role in the synthesis of unsaturated fatty acids. In this study, a complete cDNA sequence of a Δ6 desaturase-like gene was cloned from the hepatopancreas of the red claw crayfish, Cherax quadricarinatus. The full-length 1885 bp sequence comprises a 5' UTR of 254 bp, 3' UTR of 234 bp, and an open reading frame (ORF) of 1377 bp encoding a 458 amino acid polypeptide (GenBank accession no. MF497442). Bioinformatics analysis revealed three conserved histidine-rich regions, a cytochrome b5 domain at the N-terminus, and a haem binding site (HPGG) in the cytochrome b5 domain, all of which are typical of Δ6 desaturases. Quantitative real-time PCR demonstrated significantly higher expression in the hepatopancreas than other tissues. After feeding crayfish four formulated diets in which fish oil was replaced by 0, 33, 67, or 100% highly unsaturated soybean oil for 8 weeks, Δ6 desaturase-like mRNA expression and enzyme activity were higher than in the fish oil only group. Additionally, a 4-week low temperature treatment at 25, 20, 15, or 9 °C increased Δ6 desaturase mRNA expression and enzyme activity with decreasing water temperature. These results may help us better understand the biosynthesis of unsa...Continue Reading

Citations

Jun 17, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jens BoyenMarleen De Troch
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Mohd Amran Aaqillah-AmrMhd Ikhwanuddin

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