PMID: 2120196Oct 1, 1990Paper

Molecular comparison of a nonhemolytic and a hemolytic phospholipase C from Pseudomonas aeruginosa

Journal of Bacteriology
R M OstroffM L Vasil

Abstract

Pseudomonas aeruginosa produces two secreted phospholipase C (PLC) enzymes. The expression of both PLCs is regulated by Pi. One of the PLCs is hemolytic, and one is nonhemolytic. Low-stringency hybridization studies suggested that the genes encoding these two PLCs shared DNA homology. This information was used to clone plcN, the gene encoding the 77-kilodalton nonhemolytic PLC, PLC-N. A fragment of plcN was used to mutate the chromosomal copy of plcN by the generation of a gene interruption mutation. This mutant produces 55% less total PLC activity than the wild type, confirming the successful cloning of plcN. plcN was sequenced and encodes a protein which is 40% identical to the hemolytic PLC (PLC-H). The majority of the homology lies within the NH2 two-thirds of the proteins, while the remaining third of the amino acid sequence of the two proteins shows very little homology. Both PLCs hydrolyze phosphatidylcholine; however, each enzyme has a distinct substrate specificity. PLC-H hydrolyzes sphingomyelin in addition to phosphatidylcholine, whereas PLC-N is active on phosphatidylserine as well as phosphatidylcholine. These studies suggest structure-function relationships between PLC activity and hemolysis.

References

Mar 1, 1979·Microbiological Reviews·B W HollowayA F Morgan
Sep 28, 1978·Biochimica Et Biophysica Acta·S GattY Barenholz
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1986·Journal of Bacteriology·C C Grant, M L Vasil
Oct 11, 1988·Nucleic Acids Research·S E West, B H Iglewski
May 1, 1986·Infection and Immunity·M L VasilC C Grant
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D Emr, T J Silhavy
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Feb 25, 1984·Journal of Molecular Biology·G von Heijne
Sep 1, 1980·Infection and Immunity·M K Johnson, D Boese-Marrazzo
Jun 1, 1961·Journal of Bacteriology·M T ESSELMANN, P V LIU

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Citations

Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·L G RahmeF M Ausubel
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Thomas A RussoBruce A Holm
Nov 6, 2004·American Journal of Respiratory Cell and Molecular Biology·Alexis L BeattyJo Rae Wright
May 12, 2005·Current Microbiology·María J MassimelliAngela T Lisa
Jun 8, 2006·Annual Review of Microbiology·Philip A LeeGeorge Georgiou
Jan 5, 2007·The Journal of Biological Chemistry·Nozomu Okino, Makoto Ito
Oct 15, 2010·Acta Crystallographica. Section D, Biological Crystallography·S G VachieriA K Basak
Mar 5, 2017·Postȩpy higieny i medycyny doświadczalnej·Fairoz Al-WrafyAndrzej Gamian
Jun 14, 2017·Canadian Journal of Microbiology·Shawn LewenzaErik van Tilburg Bernardes
Aug 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·E J MillerA B Cohen
Oct 9, 2001·European Journal of Biochemistry·I PreussU Gehring
Nov 2, 2002·Molecular Microbiology·Martin J StonehouseMichael L Vasil
Mar 26, 2013·Applied and Environmental Microbiology·Jamie A Meadows, Matthew J Wargo
Apr 27, 2013·Pathogens and Disease·Shaan L Gellatly, Robert E W Hancock
Jun 30, 2014·Applied Microbiology and Biotechnology·Maisem LaabeiA Tobias A Jenkins
Oct 26, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T MatsuiS C Leão
Mar 29, 2003·Archives of Microbiology·Wilbert Bitter
Nov 27, 2007·Antonie van Leeuwenhoek·Marie Eliza Zamberlan da SilvaBenedito Prado Dias Filho
Jul 25, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Vinicius Vicente MartinsEliana Guedes Stehling
May 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arne RietschJohn J Mekalanos
May 3, 1996·The Journal of Biological Chemistry·T J ReillyM S Kuhlenschmidt
Jul 8, 2011·The Biochemical Journal·Angel G Díaz-SánchezRosario A Muñoz-Clares
Aug 11, 2006·The Journal of Biological Chemistry·Richard L FeltsJohn J Tanner
Jan 1, 2017·Journal of the Chinese Medical Association : JCMA·Waheed UllahNoor Muhammad
Dec 24, 2008·Infection and Immunity·Matthew J WargoDeborah A Hogan

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