Molecular components of nitrate and nitrite efflux in yeast

Eukaryotic Cell
Elisa CabreraJ M Siverio

Abstract

Some eukaryotes, such as plant and fungi, are capable of utilizing nitrate as the sole nitrogen source. Once transported into the cell, nitrate is reduced to ammonium by the consecutive action of nitrate and nitrite reductase. How nitrate assimilation is balanced with nitrate and nitrite efflux is unknown, as are the proteins involved. The nitrate assimilatory yeast Hansenula polymorpha was used as a model to dissect these efflux systems. We identified the sulfite transporters Ssu1 and Ssu2 as effective nitrate exporters, Ssu2 being quantitatively more important, and we characterize the Nar1 protein as a nitrate/nitrite exporter. The use of strains lacking either SSU2 or NAR1 along with the nitrate reductase gene YNR1 showed that nitrate reductase activity is not required for net nitrate uptake. Growth test experiments indicated that Ssu2 and Nar1 exporters allow yeast to cope with nitrite toxicity. We also have shown that the well-known Saccharomyces cerevisiae sulfite efflux permease Ssu1 is also able to excrete nitrite and nitrate. These results characterize for the first time essential components of the nitrate/nitrite efflux system and their impact on net nitrate uptake and its regulation.

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Citations

May 9, 2015·Molecular Biology and Evolution·Katie J ClowersAudrey P Gasch
Aug 12, 2014·Biochemical and Biophysical Research Communications·Chiranjit Panja, Sanjay Ghosh
Mar 9, 2019·Plants·Manuel Tejada-JimenezEmilio Fernández
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Mar 30, 2017·Frontiers in Microbiology·Andreas PfannmüllerBettina Tudzynski
Feb 16, 2017·Journal of Experimental Botany·Victoria CalatravaAurora Galvan
Jul 31, 2019·International Journal of Molecular Sciences·Lourdes RubioJosé A Fernández
Aug 17, 2019·Fungal Genetics and Biology : FG & B·E A EspesoI Larena
Oct 3, 2019·Chemical Research in Toxicology·Nichole R Johnston, Scott A Strobel
Sep 15, 2021·FEMS Yeast Research·Thomas PerliJean-Marc Daran

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