Molecular components of the exocytotic machinery in the rat pituitary gland

Endocrinology
G Jacobsson, B Meister

Abstract

Several protein components that are essential for exocytotic membrane fusion in neurons have recently been identified. The expression and cellular localization of such protein components were examined in the rat pituitary gland. In situ hybridization using isoform-specific oligonucleotide probes to different exocytotic protein messenger RNAs (mRNAs) showed strong hybridization signal for synaptotagmin I, cysteine string protein (CSP), VAMP-2 (vesicle-associated membrane protein), cellubrevin, munc-18 (mammalian homologue of unc-18), SNAP-25a (synaptosomal-associated protein of 25 kDa), syntaxin 1A, syntaxin 4, syntaxin 5, and alpha-SNAP (soluble NSF attachment protein) in the anterior and intermediate, but not in the posterior lobe of the pituitary. Moderate to weak hybridization signal was detected for synaptotagmin III, SNAP-25b, and syntaxin 2 mRNA in the anterior and intermediate, but not in the posterior lobe of the pituitary. Synaptotagmin II, VAMP-1, syntaxin 1B, or syntaxin 3 mRNA expression could not be detected in any part of the pituitary gland. Immunofluorescence histochemistry in combination with confocal laser microscopy revealed that synaptotagmin-, VAMP-, CSP-, NSF-, and alpha-SNAP-like immunoreactivities (-LI) ...Continue Reading

Citations

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