Molecular Confirmation of Intraspecific Tomato (Solanum lycopersicum) Hybrids and Their Evaluation Against Late Blight and Cucumber Mosaic Virus

Molecular Biotechnology
Amjad HameedMuhammad J Asghar

Abstract

Tomato is one of the most consumed vegetables in the world. Diseases are the number one concern in the development of high-yield and disease-resistant tomato hybrids which is the foremost priority of breeders. Present study was conducted (1) to develop DNA-based markers for genetic confirmation of tomato F1 hybrids, (2) to utilize sequenced characterized amplified region (SCAR) marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato and (3) to evaluate male and female parental genotypes and their F1 hybrids against late blight (LB) and cucumber mosaic virus (CMV). For molecular studies, 58 previously reported markers including RAPDs (10), SCAR (01), EST-SSR (01) and SSR (46) were applied. The SCAR marker clearly differentiated the LB3 and LB4 from Roma and T-1359 and provided evidence for Ph-3 gene. The SCAR marker was able to confirm the Ph-3 gene in the hybrids Roma × LB4, Roma × LB3, Riogrande × LB2, Riogrande × LB3 and Roma × LB7. Out of several tested primers, SSR-22 proved useful for genetic confirmation of F1 hybrid TMS1 × Money Maker (MM). For LB, tested hybrids/genotypes were ranked as susceptible to highly susceptible with different infection percentage (IP). However, the pace of symptom develop...Continue Reading

References

Apr 24, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chunzhi ZhangYongchen Du

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Citations

Nov 13, 2018·The Plant Journal : for Cell and Molecular Biology·Christophe RothanMathilde Causse

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