Molecular Consequences of Proprotein Convertase 1/3 (PC1/3) Inhibition in Macrophages for Application to Cancer Immunotherapy: A Proteomic Study.

Molecular & Cellular Proteomics : MCP
Marie DuhamelMichel Salzet

Abstract

Macrophages provide the first line of host immune defense. Their activation triggers the secretion of pro-inflammatory cytokines and chemokines recruiting other immune cells. In cancer, macrophages present an M2 anti-inflammatory phenotype promoting tumor growth. In this way, strategies need to be develop to reactivate macrophages. Previously thought to be expressed only in cells with a neural/neuroendocrine phenotype, the proprotein convertase 1/3 has been shown to also be expressed in macrophages and regulated as a function of the Toll-like receptor immune response. Here, we investigated the intracellular impact of the down-regulation of the proprotein convertase 1/3 in NR8383 macrophages and confirmed the results on macrophages from PC1/3 deficient mice. A complete proteomic study of secretomes and intracellular proteins was undertaken and revealed that inhibition of proprotein convertase 1/3 orient macrophages toward an M1 activated phenotype. This phenotype is characterized by filopodial extensions, Toll-like receptor 4 MyD88-dependent signaling, calcium entry augmentation and the secretion of pro-inflammatory factors. In response to endotoxin/lipopolysaccharide, these intracellular modifications increased, and the secrete...Continue Reading

References

Nov 24, 1999·The Journal of Biological Chemistry·H Q SunH L Yin
Mar 18, 2003·The Journal of Biological Chemistry·Ariel SavinaMaria I Colombo
Jan 31, 2004·Seminars in Immunology·Kiyoshi Takeda, Shizuo Akira
Jan 15, 2005·Analytical Chemistry·Jacques ColingeLydie Bougueleret
May 24, 2005·Nature Immunology·Kim A BrogdenMichael Zasloff
Oct 13, 2006·Journal of Leukocyte Biology·Marco E Bianchi
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Tadaomi Takenawa, Shiro Suetsugu
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Aug 15, 2008·Journal of Proteome Research·Wilfred H TangSean L Seymour
Sep 30, 2008·The Journal of Biological Chemistry·Mathilde ChaineauThierry Galli
Aug 12, 2009·Cytokine & Growth Factor Reviews·David Wallach, Andrew Kovalenko
Sep 3, 2010·Journal of Cellular and Molecular Medicine·Jeremy T SmythJames W Putney
Nov 4, 2010·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Dec 15, 2010·Bioinformatics·Michael E SmootTrey Ideker
Jan 21, 2011·Physiological Reviews·Alex H Hutagalung, Peter J Novick
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Jun 12, 2012·Journal of Cell Science·Anna HupalowskaMarta Miaczynska
Oct 3, 2012·PloS One·Naimish R PatelHenry Koziel
Nov 8, 2012·Nature Methods·Rintaro SaitoTrey Ideker
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Dec 12, 2012·Cancer Research·Katarzyna FranciszkiewiczFathia Mami-Chouaib
Apr 17, 2013·BMC Bioinformatics·Edward Y ChenAvi Ma'ayan
Apr 27, 2013·Science·Felix MeissnerMatthias Mann
Aug 24, 2013·Frontiers in Endocrinology·Mathilde L BonnemaisonRichard E Mains
Jan 15, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Amanda E Ward, Benjamin M Rosenthal
Apr 15, 2014·Nature Biotechnology·Juan A VizcaínoHenning Hermjakob

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Citations

Jan 13, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Adriana-Natalia MurgociMichel Salzet
Aug 12, 2017·Frontiers in Physiology·Daniele VergaraMichele Maffia
Jan 25, 2021·The Science of the Total Environment·Mateusz OlejnikMatthias Epple
Dec 11, 2020·Scientific Reports·Markus KerstingMatthias Epple

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