Molecular coordination of Staphylococcus aureus cell division.

ELife
Victoria A LundSimon J Foster

Abstract

The bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple interacting components. The human pathogen Staphylococcus aureus is a prolate spheroid that divides in three orthogonal planes. Here, we have integrated cellular morphology during division with molecular level resolution imaging of peptidoglycan synthesis and the components responsible. Synthesis occurs across the developing septal surface in a diffuse pattern, a necessity of the observed septal geometry, that is matched by variegated division component distribution. Synthesis continues after septal annulus completion, where the core division component FtsZ remains. The novel molecular level information requires re-evaluation of the growth and division processes leading to a new conceptual model, whereby the cell cycle is expedited by a set of functionally connected but not regularly distributed components.

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Citations

Jul 11, 2019·Molecular Microbiology·Martin S BojerHanne Ingmer
Jun 12, 2020·Nature·L Pasquina-LemoncheJ K Hobbs
May 16, 2019·Nature Microbiology·Nathalie T ReichmannMariana G Pinho
Mar 7, 2020·Scientific Reports·Claes Søndergaard WassmannJanne Kudsk Klitgaard
Mar 13, 2021·Molecular Microbiology·Daniel StraumeLeiv Sigve Håvarstein
Dec 30, 2020·Chemical Reviews·Jed F Fisher, Shahriar Mobashery
Jun 26, 2021·Methods : a Companion to Methods in Enzymology·Mark C Leake
Oct 31, 2021·Proceedings of the National Academy of Sciences of the United States of America·Bartłomiej SalamagaSimon J Foster

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Methods Mentioned

BETA
imaging technique
fluorescence microscopy
AFM
diffraction-limited microscopy
PCR
electron microscopy

Software Mentioned

Nikon elements
CoPro
Matlab
Matlab scripts ImageJ
SIM
ThunderSTORM ImageJ
Fiji
Image J / Fiji
ADA
DA

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Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.