Molecular crowding effects on protein stability

Annals of the New York Academy of Sciences
Florin DespaRaphael C Lee

Abstract

The volume fraction occupied by the dry matter of the cell can be as large as 40%, of which more than half (approximately 60%) are proteins. Thus, cellular proteins and protein assemblies occupy a large volume that can have a profound effect on their own native-state stabilities and on their unfolding/refolding rates. In addition, macromolecular crowding can change the properties of a significant fraction of the water in the cell. We review features of the molecular crowding effect which are relevant for describing the microscopic mechanism of thermal injuries.

References

Dec 6, 1995·Biochimica Et Biophysica Acta·I V DudichT Korpela
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Dec 23, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·M B Burg
Feb 17, 2001·Current Opinion in Structural Biology·R J Ellis
Feb 14, 2002·Trends in Biochemical Sciences·Alan S Verkman
May 31, 2002·Nature·David Chandler
Jul 25, 2003·Biochimica Et Biophysica Acta·Damien Hall, Allen P Minton
Apr 9, 2004·Journal of the American Chemical Society·Robert C FordJichen Li
Dec 17, 2004·Physical Review Letters·Florin DespaR Stephen Berry
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Margaret S CheungD Thirumalai
Jul 5, 2005·Burns : Journal of the International Society for Burn Injuries·F DespaR C Lee
Sep 1, 2005·Annals of Biomedical Engineering·Florin DespaRaphael C Lee

❮ Previous
Next ❯

Citations

Sep 17, 2013·Journal of Molecular Graphics & Modelling·Michael Feig, Yuji Sugita
Feb 23, 2012·Journal of the American Chemical Society·Ryuhei HaradaMichael Feig
Nov 13, 2008·Nature Reviews. Molecular Cell Biology·Shruthi S Vembar, Jeffrey L Brodsky
Dec 17, 2009·Nucleic Acids Research·Renaud GeslainLluís Ribas de Pouplana
Feb 20, 2008·Biochemistry. Biokhimii︠a︡·N A Chebotareva
Jul 17, 2010·Biochemistry. Biokhimii︠a︡·I P BaskovaA S Dudkina
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Carol B FowlerJeffrey T Mason
Jul 16, 2010·Meat Science·E Puolanne, Marjo Halonen
Jun 26, 2007·Protein Science : a Publication of the Protein Society·Tomoo OhashiHarold P Erickson
Dec 7, 2010·Journal of Diabetes Science and Technology·Florin Despa, R Stephen Berry
Dec 25, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Constantin Ionescu-Tirgoviste, Florin Despa
Aug 19, 2008·Journal of Immigrant and Minority Health·Katherine StandishSandro Galea
Oct 18, 2019·Methods and Applications in Fluorescence·Nguyen N H TranAndrew Clayton
May 22, 2016·The Biochemical Journal·Daniel FonsecaGonçalo da Costa
Feb 27, 2013·Chemical Communications : Chem Comm·Yousuke TakaokaItaru Hamachi
Jun 3, 2020·Cold Spring Harbor Protocols·Clara L KielkopfIna L Urbatsch
Aug 20, 2021·The Journal of Physical Chemistry. B·Akihisa MiyagawaKiyoharu Nakatani
Oct 3, 2012·The Journal of Physical Chemistry. B·Ashima MalikPramit K Chowdhury
Nov 21, 2017·The Journal of Physical Chemistry. B·Grzegorz NawrockiMichael Feig
Sep 27, 2021·Comprehensive Reviews in Food Science and Food Safety·Yulong BaoRuichang Gao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.