PMID: 9193955May 1, 1997Paper

Molecular delineation of Cylicocyclus nassatus and C. ashworthi (Nematoda:Strongylidae)

International Journal for Parasitology
G C HungR B Gasser

Abstract

The nucleotide sequences of the first internal transcribed spacer (ITS-1), 5.8S gene and second internal transcribed spacer (ITS-2) of ribosomal DNA have been determined for Cylicocyclus nassatus, C. ashworthi and C. insignis. Pairwise comparisons revealed sequence differences between the taxa ranging from 3.8 to 6.2% for the ITS-2 and 2.2-2.7% for the ITS-1. For the ITS-1, the level of the sequence difference between C. ashworthi and C. nassatus (2.2%) was equivalent to that between C. nassatus and C. insignis (2.2%), indicating that C. ashworthi and C. nassatus represent separate species. Theoretical restriction maps were constructed from the sequence data, and a polymerase chain reaction-linked restriction fragment length polymorphism (PCR-linked RFLP) technique was established to unequivocally distinguish C. ashworthi from C. nassatus.

References

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Citations

Nov 26, 2010·Parasitology Research·Tetiana A KuzminaEugene T Lyons
Mar 8, 2000·International Journal for Parasitology·L van HerwerdenD Blair
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Oct 7, 2003·International Journal for Parasitology·J E HodgkinsonJ B Matthews
Feb 19, 1998·International Journal for Parasitology·R B Gasser
Mar 26, 1998·International Journal for Parasitology·L A NewtonR B Gasser
Mar 26, 1998·International Journal for Parasitology·L A NewtonR B Gasser
May 29, 1998·International Journal for Parasitology·A RomstadN B Chilton
Apr 30, 1999·International Journal for Parasitology·G C HungR B Gasser
May 20, 1999·International Journal for Parasitology·X ZhuR B Gasser
Feb 17, 2000·International Journal for Parasitology·G C HungR B Gasser
Mar 25, 2000·International Journal for Parasitology·M Blaxter
Jul 10, 1998·Molecular and Biochemical Parasitology·R B GasserN B Chilton
Oct 2, 2003·Veterinary Parasitology·M R ChapmanT R Klei
Aug 24, 1999·Veterinary Parasitology·R B Gasser
Sep 15, 1999·Veterinary Parasitology·S LoveD Mellor
Oct 3, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·E AbolloS D'Amelio
Dec 1, 1999·International Journal for Parasitology·R B Gasser, P O'Donoghue
Jun 20, 2002·Veterinary Parasitology·Jane L KinsellaMichael F Ryan
Jun 12, 2001·Molecular Phylogenetics and Evolution·J Gouÿ de BellocqM C Durette-Desset
May 1, 2019·Parasites & Vectors·Muhammad A SaeedAbdul Jabbar
Jul 14, 2017·Frontiers in Cellular and Infection Microbiology·Christina M BredtmannGeorg von Samson-Himmelstjerna

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