PMID: 9192672Jun 24, 1997Paper

Molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases to 1-1.5 Mb and preparation of a PAC-based physical map

Proceedings of the National Academy of Sciences of the United States of America
N ZhaoM M Le Beau

Abstract

Loss of a whole chromosome 5 or a deletion of the long arm, del(5q), is a recurring abnormality in malignant myeloid diseases. In previous studies, we delineated a commonly deleted segment of approximately 4 Mb within band 5q31 that was flanked by IL9 on the proximal side and D5S166 on the distal side. We have generated a physical map of P1 (PAC), bacterial (BAC), and yeast artificial chromosome (YAC) clones of this interval. The contig consists of 108 clones (78 PACs, 2 BACs, and 28 YACs) to which 125 markers (5 genes, 11 expressed sequence tags, 12 polymorphisms, and 97 sequence-tagged sites) have been mapped. Using PAC clones for fluorescence in situ hybridization analysis of leukemia cells with a del(5q), we have narrowed the commonly deleted segment to 1-1.5 Mb between D5S479 and D5S500. To search for allele loss, we used 7 microsatellite markers within and flanking the commonly deleted segment to examine leukemia cells from 28 patients with loss of 5q, and 14 patients without cytogenetically detectable loss of 5q. In the first group of patients, we detected hemizygous deletions, consistent with the cytogenetically visible loss; no homozygous deletions were detected. No allele loss was detected in patients without abnormal...Continue Reading

References

Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K SadhuP Russell
Jul 1, 1985·Cancer Genetics and Cytogenetics·H Van den BergheG Tricot
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·J FairmanL Nagarajan
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A G Knudson
Nov 10, 1994·Nature·T H Rabbitts
Jan 1, 1993·Annual Review of Biochemistry·A J Levine
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M M Le BeauC A Westbrook
Apr 3, 1996·Journal of the National Cancer Institute·M A SmithJ E Karp
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·M StoffelG I Bell
Jul 1, 1996·Nature Genetics·G J RigginsB Vogelstein
Apr 1, 1996·Hematology/oncology Clinics of North America·M J Thirman, R A Larson
Oct 25, 1996·Science·G D SchulerT J Hudson

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Citations

Jun 13, 1998·Genes, Chromosomes & Cancer·R J JajuL Kearney
Jan 25, 2002·International Journal of Cancer. Journal International Du Cancer·Katalin CsiszarCharles D Boyd
Jan 13, 2000·Biochemical and Biophysical Research Communications·Y WangM A Hediger
Oct 4, 2011·Current Treatment Options in Oncology·Eric PadronAlan F List
Jun 7, 2005·Cancer Letters·Anna D Panani, Charis Roussos
May 9, 2002·Cancer Genetics and Cytogenetics·Christèle DubourgLucienne Guenet
Mar 29, 2003·Regulatory Toxicology and Pharmacology : RTP·Richard AlbertiniCurtis Travis
Feb 5, 1999·Gene·K L StoddartN T Bech-Hansen
Jan 23, 1999·Current Opinion in Cell Biology·C T Jordan, G Van Zant
Oct 29, 2000·British Journal of Haematology·C A WestbrookS K Horrigan
Apr 18, 2002·Genes & Development·Bhuvanesh SinghArchontoula Stoffel
Jun 1, 1997·Immunological Reviews·B R BlazarD A Vallera
Feb 11, 2014·Best Practice & Research. Clinical Haematology·Rami S KomrokjiAlan F List
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhijian QianMichelle M Le Beau
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ting Xi LiuA Thomas Look
Mar 15, 2014·Annals of Hematology·Mahmoud R Gaballa, Emmanuel C Besa
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Aug 26, 1998·Leukemia & Lymphoma·P D CastroL Nagarajan
Sep 29, 2011·Seminars in Oncology·Benjamin L Ebert
Nov 29, 2011·Clinics in Laboratory Medicine·Olatoyosi OdenikeMichelle M Le Beau
Dec 7, 2010·Best Practice & Research. Clinical Haematology·Benjamin L Ebert
Dec 2, 2008·Best Practice & Research. Clinical Haematology·Jasmine C WongKevin Shannon
Nov 26, 2008·Cancer Genetics and Cytogenetics·Claudia HaferlachAlan List
Aug 12, 2008·Seminars in Oncology·Lucy A Godley, Richard A Larson
Jan 26, 2008·British Journal of Haematology·Charikleia KelaidiPierre Fenaux

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