Molecular detection and immunopathological examination of Deltapapillomavirus 4 in skin and udder of Egyptian cattle

Veterinary World
Emad Beshir AtaA A Madboli

Abstract

Bovine papillomaviruses (BPVs) are the main cause of bovine papillomatosis resulting in cutaneous and/or mucosal benign tumors that could be transformed to malignant ones with marked economic importance, especially in the dairy farms. Molecular, pathological, and immunohistochemical (IHC) diagnosis of bovine papillomatosis cases was conducted to identify and characterize the circulating BPV genotype in some Egyptian governorates. Wart-like lesions in skin, udder, and teats were collected from 123 infected cases in Giza, Beni Suef, and El Menoufia Governorates, Egypt, during 2016-2017. Pathological and IHC characterization, molecular identification, genotyping, and phylogenetic analysis based on the conserved late (L1) gene of the all samples were carried out. 89 of the 123 collected samples (72.3%) were positively detected by polymerase chain reaction (PCR). The sequence analysis of the obtained PCR amplicons was identical revealing identification and genotyping of only one type (Deltapapillomavirus 4 isolate EGY 2017) with accession number (MG547343) which found to be closely related to the recently detected Deltapapillomavirus 4 isolate 04_asi_UK (accession no. MF384288.1) and isolate Deltapapillomavirus 4 isolate 25_equ_CH (...Continue Reading

References

Sep 16, 2014·Molecular Medicine·Nilesh M Agalave, Camilla I Svensson

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Citations

Jul 4, 2019·Transboundary and Emerging Diseases·Hao PengShiwen Feng
Mar 10, 2020·Veterinary Medicine International·Karima A Al-SalihiTho Alfiqar H Ali
Feb 14, 2021·Tropical Animal Health and Production·Emad Beshir AtaMohamed Abd El-Fatah Mahmoud
Oct 25, 2020·Animals : an Open Access Journal From MDPI·Mohamed El-TholothEhab Kotb Elmahallawy

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Methods Mentioned

BETA
biopsies
PCR
genotyping

Software Mentioned

Basic Local Alignment Search
Bioedit

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