Molecular detection and phylogenetic characterization of Gordonia species in heavily oil-contaminated soils

Research in Microbiology
Fo-Ting ShenChiu-Chung Young

Abstract

This study was undertaken to assess genetic diversity among Gordonia species present in heavily oil-contaminated sites using both a culture-dependent and a culture-independent (PCR-denaturing gradient gel electrophoresis (DGGE)) approach. Soil samples for this purpose were collected from 8 different heavily (crude) oil-contaminated industrial park sites located around Kaohsiung County, Taiwan. Using Gordonia-specific PCR-DGGE, a significant increase in Gordonia species diversity was noted in 1% heavily oil-enriched soil. A total of 67 strains were scored and identified as Gordonia after genus-specific PCR amplification and sequencing. BOX-PCR fingerprinting of culturable Gordonia showed wide strain diversity. A total of 33 different strains were identified from most of the sampling sites. Based on gyrB gene sequence analysis, all Gordonia strains could be segregated into five major clusters. Gordonia amicalis was the predominant species in all oil-amended soil samples. Isolates sharing <98.5% gyrB gene sequence similarities with Gordonia type strains represent indigenous novel Gordonia species. Variations in phenotypic characteristics further confirm the presence of a wide range of species and strain diversity among Gordonia is...Continue Reading

References

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Jun 19, 2007·Bioresource Technology·Rodrigo J S JacquesFlavio A O Camargo

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Citations

Mar 28, 2012·The Journal of General and Applied Microbiology·Jirapat Chanthamalee, Ekawan Luepromchai
Jan 6, 2010·Systematic and Applied Microbiology·Fo-Ting ShenChiu-Chung Young
May 12, 2009·Systematic and Applied Microbiology·Fo-Ting ShenChiu-Chung Young
Jan 14, 2012·Microbiological Research·Chun-Liang LinChiu-Chung Young
Mar 1, 2017·International Journal of Environmental Research and Public Health·Yi-Bin QiCheng-Gang Zheng

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