Molecular detection and prevalence of enterovirus within environmental water in Taiwan

Journal of Applied Microbiology
C-H ChenM-T Wan

Abstract

To investigate the presence of enterovirus RNA in various samples of environmental water collected in Taiwan during 2004-2005, and to characterize the genotypes and distribution of the viruses identified in Taiwan. Total 131 environmental samples were screened using the reverse transcription polymerase chain reaction (RT-PCR) for the highly conserved 5'-nontranslated regions (5'-NTR). Among these enterovirus RNA-positive samples, 32, 15 and 6 of the samples were recovered from surface water, ground water and sewage water respectively. However, the total positive detection rate increased to 40.5% with the application of seminested PCR. Sequencing revealed that the majority of isolates belonged to the following genotypes: coxsackie A2 (35.8%), coxsackie A6 (13.2%) and enterovirus (EV)71 (11.3%); echovirus 11, porcine EV9 and coxsackie A16 isolates were also observed. This study confirms that the major epidemic genotypes of enterovirus are prevalent in the surface and ground water of Taiwan. This study is helpful in understanding the significance and epidemiology of the virus within and beyond the study area. Moreover, it was possible to predict the enterovirus genotype and evaluate possible correlations between water contaminatio...Continue Reading

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Citations

Jan 9, 2010·Epidemiology and Infection·S S Y WongK Y Yuen
Aug 20, 2010·The Journal of Antimicrobial Chemotherapy·Tak-Wah WongChun-Keung Yu
Mar 10, 2009·Applied and Environmental Microbiology·Jens VerheyenHerbert Pfister
Apr 29, 2011·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chien-Hui HsuLi-Min Huang
Feb 13, 2013·The Journal of Hospital Infection·S-C ChangT-Y Lin
Nov 27, 2015·Journal of Water and Health·Johnson Lin, Atheesha Singh

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