Molecular Detection of Borrelia burgdorferi Sensu Lato and Anaplasma phagocytophilum in Ticks Collected from Dogs in Urban Areas of North-Eastern Poland

Pathogens
Mirosław M MichalskiMałgorzata Dmitryjuk

Abstract

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6-35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1-40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9-22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3-2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4-2.9%). In 85.4% (141/165, 95% CI: 79.1-90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5-20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

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Citations

Jun 3, 2021·Pathogens·Mirosław M MichalskiMałgorzata Dmitryjuk

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Prism
BLASTn
GraphPad

Related Concepts