Molecular detection of Coxiella burnetii in livestock farmers and cattle from Magdalena Medio in Antioquia, Colombia

PloS One
Ruth Cabrera OrregoLina Andrea Gutiérrez

Abstract

Coxiella burnetii causes Q fever in humans and coxiellosis in animals. In humans, it causes acute febrile illnesses like influenza, pneumonia, hepatitis, and chronic illnesses such as endocarditis, vascular infection, and post-infectious fatigue syndrome. It is widely distributed worldwide, and its main reservoirs are sheep, goats, and cattle. This study aimed to determine the frequency of C. burnetii infection using molecular detection and to identify the associated factors in livestock farmers and cattle from the Magdalena Medio region of Antioquia, Colombia. Using real-time polymerase chain reaction (PCR), molecular detection was performed for the IS1111 insertion sequence of C. burnetii using genomic DNA collected from the peripheral blood of 143 livestock farmers and 192 cattle from 24 farms located in Puerto Berrío, Puerto Nare, and Puerto Triunfo. To confirm the results, bidirectional amplicon sequencing of 16S rRNA was performed in four of the positive samples. Additionally, factors associated with C. burnetii were identified using a Poisson regression with cluster effect adjustment. Real-time PCR showed positive results in 25.9% and 19.5% of livestock farmer samples and cattle samples, respectively. For livestock farme...Continue Reading

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Citations

Apr 13, 2021·Vector Borne and Zoonotic Diseases·Hyung-Chul ChoKyoung-Seong Choi
Jul 27, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Igor Rosa MeurerJosé Otávio do Amaral Corrêa

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Datasets Mentioned

BETA
MN540443

Methods Mentioned

BETA
PCR

Software Mentioned

BLASTn
Geneious®
SPSS
Stata
Interactive Tree Of Life ( iTOL )
PhyML
jModelTest
ArcGIS®

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