Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Veterinary Medicine and Science
Muhammad Irfan MalikF Iqbal

Abstract

Canine monocytic ehrlichiosis is a tick-borne disease caused by an intracellular alpha-proteobacterium, Ehrlichia canis, which replicates within mononuclear cells in the host. This study was designed to use a polymerase chain reaction (PCR) protocol for the molecular detection of E. canis by the amplification of a portion of its 16S rRNA gene, as well as the effects of this alpha-proteobacterium on the haematological parameters of the sampled dogs and the risk factors associated with E. canis infection. A total of 151 blood samples were collected from dogs of various breeds at three sampling sites (Lahore, Rawalpindi/Islamabad and Multan) in Punjab, Pakistan. Data regarding the epidemiological factors (including age, gender, breed, body temperature, deworming, vaccination, mucous membrane status, hydration status, the presence of haematuria and tick infestation) were collected through a questionnaire at the time of sample collection. A 400 bp DNA fragment of the 16S rRNA gene of E. canis was amplified from 42 dog blood samples (28% of the total), [Lahore (N = 24), Rawalpindi/Islamabad (N = 13) and Multan (N = 05)] through PCR. Data analysis revealed that the character of the animals (age, sex and breed) had no significant assoc...Continue Reading

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Citations

Sep 12, 2018·PLoS Neglected Tropical Diseases·Faham KhamesipourEhsan Rakhshandehroo
Jan 5, 2020·Parasites & Vectors·Abdul GhafarAbdul Jabbar
Jan 29, 2021·Parasitology Research·Roberta IattaDomenico Otranto
Apr 13, 2021·The Journal of Parasitology·Hammad Nayyar GhauriAwais Ghaffar
May 25, 2021·Veterinary Parasitology : Regional Studies and Reports·Moran RahamimItamar Aroch
Oct 17, 2020·Parasites & Vectors·Carlos A Rodríguez-AlarcónJulio V Figueroa-Millán

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Minitab

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