Abstract
Canine monocytic ehrlichiosis is a tick-borne disease caused by an intracellular alpha-proteobacterium, Ehrlichia canis, which replicates within mononuclear cells in the host. This study was designed to use a polymerase chain reaction (PCR) protocol for the molecular detection of E. canis by the amplification of a portion of its 16S rRNA gene, as well as the effects of this alpha-proteobacterium on the haematological parameters of the sampled dogs and the risk factors associated with E. canis infection. A total of 151 blood samples were collected from dogs of various breeds at three sampling sites (Lahore, Rawalpindi/Islamabad and Multan) in Punjab, Pakistan. Data regarding the epidemiological factors (including age, gender, breed, body temperature, deworming, vaccination, mucous membrane status, hydration status, the presence of haematuria and tick infestation) were collected through a questionnaire at the time of sample collection. A 400 bp DNA fragment of the 16S rRNA gene of E. canis was amplified from 42 dog blood samples (28% of the total), [Lahore (N = 24), Rawalpindi/Islamabad (N = 13) and Multan (N = 05)] through PCR. Data analysis revealed that the character of the animals (age, sex and breed) had no significant assoc...Continue Reading
References
Jul 1, 1994·Journal of Clinical Microbiology·Z IqbalY Rikihisa
Oct 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J W McBrideB I Osburn
Jul 1, 1997·Journal of Clinical Microbiology·B WenR Bartsch
Mar 24, 2004·Veterinary Parasitology·Márcio Botelho de CastroMirela Tinucci Costa
Dec 28, 2004·Veterinary Parasitology·R I Rodriguez-VivasG M E Bolio
Feb 19, 2008·Veterinary Parasitology·Filipe Dantas-Torres
Feb 22, 2008·Veterinary Parasitology·A ShipovS Harrus
Mar 17, 2010·The Veterinary Journal·Shimon Harrus, Trevor Waner
Apr 10, 2010·Parasites & Vectors·Sd GauntEb Breitschwerdt
Jul 20, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S HarrusG Baneth
Sep 3, 2010·Veterinary Parasitology·Paula Virgínia GomesMárcia Cristina Cury
Apr 1, 2011·Parasitology Research·S ShahnawazFurhan Iqbal
Apr 15, 2011·Parasites & Vectors·Domenico OtrantoGad Baneth
Jul 21, 2011·Parasites & Vectors·Puteri Azaziah Megat Abd RaniRebecca J Traub
Nov 24, 2011·Ticks and Tick-borne Diseases·Andréia L T MeloDaniel M Aguiar
May 23, 2012·Vector Borne and Zoonotic Diseases·Viorica MirceanVasile Cozma
Nov 22, 2012·Ticks and Tick-borne Diseases·Adrian P YbañezHisashi Inokuma
Nov 22, 2012·Ticks and Tick-borne Diseases·Hiromi SasakiHisashi Inokuma
Jan 10, 2013·PLoS Neglected Tropical Diseases·Mojgan NazariMalaika Watanabe
Jan 22, 2013·PloS One·Patrick J KellyChengming Wang
Mar 19, 2013·Parasitology International·Seung Won KangKi Yoon Chang
Mar 29, 2013·Parasitology Research·Munir AktasDuygu Neval Sayın Ipek
Dec 10, 2013·Veterinary Parasitology·Alicia RojasGad Baneth
Jan 16, 2014·Veterinary Parasitology·Munir Aktas
Jul 21, 2014·Research in Veterinary Science·R L de MirandaG Baneth
Apr 19, 2015·Parasites & Vectors·Munir AktasNazir Dumanlı
May 11, 2016·Parasites & Vectors·Tawin InpankaewRebecca J Traub
Citations
Sep 12, 2018·PLoS Neglected Tropical Diseases·Faham KhamesipourEhsan Rakhshandehroo
Sep 13, 2019·Parasites & Vectors·Babette AbandaAlbert Eisenbarth
Jul 9, 2020·Veterinary World·Qiao MengfanMeng Qingling
Jan 5, 2020·Parasites & Vectors·Abdul GhafarAbdul Jabbar
Jan 29, 2021·Parasitology Research·Roberta IattaDomenico Otranto
Apr 13, 2021·The Journal of Parasitology·Hammad Nayyar GhauriAwais Ghaffar
May 25, 2021·Veterinary Parasitology : Regional Studies and Reports·Moran RahamimItamar Aroch
Oct 17, 2020·Parasites & Vectors·Carlos A Rodríguez-AlarcónJulio V Figueroa-Millán