Molecular detection of GES-2 extended spectrum Beta-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa

International Journal of Antimicrobial Agents
Gerhard F Weldhagen, Andrea Prinsloo

Abstract

Screening for and detection of the novel extended spectrum Beta-lactamase (ESBL), GES-2 produced by Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for P. aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371 bp segment of bla(GES-2). This should lead to more cost-effective DNA sequencing and sequence interpretation in the laboratory.

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Citations

Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Aurora García-FernándezAlessandra Carattoli
Jan 19, 2012·Antimicrobial Agents and Chemotherapy·Aurora García-FernándezAlessandra Carattoli
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Oct 15, 2005·Clinical Microbiology Reviews·David L Paterson, Robert A Bonomo
Jul 13, 2012·Journal of Clinical Microbiology·Martin KaaseSören G Gatermann
Oct 12, 2010·Journal of Global Infectious Diseases·Deepti Rawat, Deepthi Nair
Dec 18, 2009·Expert Review of Anti-infective Therapy·Alexandre P ZavasckiAna C Gales
Dec 4, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Sz-Rung HuangZhi-Yuan Shi
Mar 1, 2012·Enfermedades infecciosas y microbiología clínica·Maribel González-VillaGerardo Aparicio-Ozores
Jun 17, 2009·Journal of Medical Microbiology·Tanya Strateva, Daniel Yordanov
Jul 28, 2013·SpringerPlus·Marketa SenkyrikovaMilan Kolar

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