Molecular Detection of Human Astrovirus in Children With Gastroenteritis, Northern Italy

The Pediatric Infectious Disease Journal
Massimiliano BergalloPier-Angelo Tovo

Abstract

Human astroviruses have increasingly been identified and are important agents of diarrheal disease, especially in infants and young children. This article presents the real-time polymerase chain reaction TaqMan assay for the detection and quantification of human astrovirus for clinical fecal samples collected from hospitalized children with acute gastroenteritis in Piedmont (northern Italy) from December 2014 to November 2015. A total of 159 fecal specimens from hospitalized children with acute gastroenteritis, previously screened for rotavirus, adenovirus, norovirus, human parechovirus, salivirus and sapovirus, were tested for human astrovirus. The most commonly detected virus was norovirus GII (33.8%), followed by rotavirus (21.3%), sapovirus (10.9%), human parechovirus (8%), norovirus GI (6.7%), adenovirus (1%) and salivirus (0.52%). A total of 30 of 159 (18.87%) episodes of acute gastroenteritis were associated with human astrovirus genomic detection. Our data showed that the detection rate of astrovirus in diarrheal children (18.87%) was higher than observed in other countries, where they were reported in diarrheal children in 10.3%-0.8% of patients and a mean incidence worldwide of 11%. Our data showed that the detection ...Continue Reading

References

Feb 5, 2004·Research in Microbiology·Pierre Le CannVirginie Ferré
Jun 6, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Wei-xia ChengZhao-yin Fang
Apr 22, 2009·PLoS Pathogens·Jane L ArthurRodney M Ratcliff
May 21, 2009·Journal of Clinical Laboratory Analysis·Marijke RaymaekersReinoud Cartuyvels
Jun 23, 2009·Virology Journal·Alexander L GreningerJoseph L DeRisi
Aug 4, 2010·Emerging Infectious Diseases·Tongling ShanXiuguo Hua
Sep 11, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Noortje M van MaarseveenEric C J Claas
Jun 7, 2011·Archives of Virology·Sara Giordana RimoldiMaria Rita Gismondo
Jan 3, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Wei-Chen TsengYhu-Chering Huang
Jan 1, 2012·African Journal of Infectious Diseases·Ci AyolabiI Akpan
Mar 14, 2014·Annals of Laboratory Medicine·Heejin HamSonil Pak
Oct 4, 2014·Clinical Microbiology Reviews·Albert BoschSusana Guix
Mar 23, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Sonia Etenna Lekana-DoukiEric M Leroy
Apr 17, 2015·Journal of Medical Virology·Aksara ThongprachumHiroshi Ushijima
Apr 19, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N SantosD P Lima
Jun 14, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Massimiliano BergalloPaolo Ravanini
Sep 13, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Souhaib AldabbaghAnna M Eis-Hübinger
Nov 5, 2016·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Krishna Chaaithanya IttaSubarna Roy
Nov 4, 2016·Open Forum Infectious Diseases·Jumi YiEvan J Anderson
Nov 7, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Massimiliano BergalloClara Gabiano
Jan 14, 2017·Viruses·Philippe PérotMarc Eloit

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.