Molecular detection of human papillomavirus and viral DNA load after radiotherapy for cervical carcinomas

Tumori
Saloua KahlaRidha Oueslati

Abstract

Infection with high-risk types of human papillomavirus (HPV) is a necessary cause for cervical carcinoma. Radiation therapy together with surgery is the most effective treatment. The purpose of this study was to investigate the molecular basis of the response to radiotherapy in cervical cancer cells. Tumor cells were obtained from biopsies of 44 cervical cancers, collected before and after radiotherapy. The presence of HPV was analyzed by polymerase chain reaction (PCR) using primers specific for the L1 region. The prevalence of HPV was 70.4%, with HPV16 being the most common (54.5%) and HPV18 the second (15.9%). Our analyses show that ionizing radiation does not influence HPV detection, as the percentage of HPV-positive biopsies was similar in patients before and after radiotherapy (HPV16 60% vs. 51.7% and HPV18 20% vs. 13.7%, respectively). However, the detection of HPV did vary by tumor stage, with the highest proportion observed in late-stage tumors (HPV16 and HPV18 in 80% and 60% of stage III tumors, respectively). We also found that HPV viral load is influenced by radiotherapy and tumor stage, with the highest viral loads in late-stage tumors (stage III) after 1 day since radiotherapy (p<0.05). According to Kaplan-Meie...Continue Reading

References

Jun 22, 1995·International Journal of Cancer. Journal International Du Cancer·J CzeglédyG Wadell
Jun 26, 2001·International Journal of Experimental Pathology·H R McMurrayD J McAnce
Mar 29, 2002·Journal of Clinical Pathology·F X BoschK V Shah
Jan 31, 2003·British Journal of Cancer·G M CliffordS Franceschi
Feb 5, 2004·British Journal of Cancer·J O ThomasS Franceschi
Apr 6, 2004·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Mongi MaalejKhaled Ben Romdhane
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Nubia MuñozChris J L M Meijer
Dec 14, 2004·American Journal of Obstetrics and Gynecology·Yutaka NagaiKoji Kanazawa
Mar 1, 2006·BMC Infectious Diseases·Luis Francisco Sánchez-AnguianoMargarita Carrera-Rodríguez
May 10, 2006·International Journal of Radiation Oncology, Biology, Physics·Katja LindelGuenther Gruber
Jun 29, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·R K SinghC K Panda
Aug 8, 2006·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Aslaug HellandAnne-Lise Børresen-Dale
Sep 16, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sonia AnderssonBo Johansson
Apr 27, 2010·Cancer Treatment Reviews·Marie-Catherine VozeninEric Deutsch
Nov 11, 2010·International Journal of Cancer. Journal International Du Cancer·Yong Jung SongSang-Yoon Park
Nov 28, 2012·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·F Le TinierP Nickers
Oct 31, 2013·The International Journal of Biological Markers·Saloua KahlaRidha Oueslati
Jan 3, 2014·BioMed Research International·Maria Lina TorneselloFranco M Buonaguro
Apr 26, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Saloua KahlaRidha Oueslati
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal

❮ Previous
Next ❯

Methods Mentioned

BETA
hysterectomy
biopsies
biopsy
electrophoresis
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
K LindelG Gruber
International Journal of Cancer. Journal International Du Cancer
E V SamoylovaF L Kisseljov
Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Saloua KahlaRidha Oueslati
© 2021 Meta ULC. All rights reserved